PreScission Protease is a genetically engineered fusion protein of human rhinovirus 3C protease and glutothione S trasferase (GST). PreScisson protease specifically cleaves between the Gln and Gly residues of the recognition sequence LeuGluValLeuPheGln/GlyPro.
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Cat. No.: PSP-100 (for 100U)
Cat. No.: PSP-500 (for 500U)
PreScission Protease specifically recognizes Gln and Gly residues in the short peptide Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro for cleavage. The recognition and cleavage of substrates depend not only on the primary structure of the fusion protein but also on the secondary and tertiary structure. It can specifically separate GST tags with enzyme substrate recognition polypeptide sequence fusion proteins expressed by vectors such as pGEX-6P series. The source of this protein is the recombinant expression of E. coli.
The amount of enzyme required to cleave more than 90% of 100 μg GST-tagged fusion protein after 16 hours of reaction at 5 °C is defined as an active unit.
PreScission Protease can be stored for 2 years at -80 °C for a long time or for 6 months at -20 °C after sub-packaged to avoid repeated freezing and thawing. 10 x PreScission Buffer can be stored at -20 °C.