Mouse Intestinal Lamina Propria Immune Cell Separation Kit
$650.00 - $2,400.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: MILPIC-10 (for 10T)
Cat. No.: MILPIC-50 (for 50T)
Description
Mouse Intestinal Lamina Propria Immune Cell Separation Kit, also referred to as the Mouse Intestinal Tissue Dissociation Kit or Lamina Propria Dissociation Kit, enables rapid and high-yield isolation of lamina propria immune cells (including lymphocytes and myeloid cells) from mouse small and large intestinal tissues (colon and rectum included). Its working principle combines enzymatic digestion of extracellular matrix adhesion proteins with mild mechanical dissociation. Single-cell suspensions prepared with this kit exhibit excellent cell viability and high recovery efficiency, while preserving intact cell surface epitopes, making them suitable for downstream applications such as primary cell culture, cell sorting, and single-cell RNA sequencing. This kit must be used in conjunction with a cell density gradient separation medium (Density Gradient Media).
The intestinal lamina propria serves as the central hub for intestinal mucosal immune responses, housing diverse immune cell populations of lymphoid and myeloid lineages. Modulated by the intestinal microenvironment, lamina propria T lymphocytes differentiate into effector subsets (e.g., Th17 cells) and regulatory T cells (Tregs). Meanwhile, local B lymphocytes mature into plasma cells that secrete secretory immunoglobulin A (sIgA), which collectively maintain intestinal mucosal barrier integrity and immune homeostasis. Myeloid-derived populations primarily consist of phagocytic macrophages and antigen-capturing, antigen-presenting dendritic cells (DCs).
Through intricate cytokine signaling networks and cell-surface molecular crosstalk, intestinal lamina propria immune cells construct a specialized mucosal immune niche. This microenvironment efficiently eliminates invasive pathogens while mediating immune tolerance to commensal microbes, jointly sustaining intestinal immune equilibrium. Accordingly, the isolation and characterization of lamina propria immune cells are indispensable for dissecting the molecular mechanisms governing intestinal immune homeostasis and related regulatory pathways.
Components
- Digestion Buffer A
- Supplement Reagent A (500X)
- Digestion Buffer B
- Enzyme I (500X)
- Enzyme II
- Digestion Stop Reagent (250X)
Workflow
This kit facilitates gentle, rapid, and efficient extraction of lamina propria lymphocytes and myeloid immune cells from mouse intestinal tissue via a three-step workflow:
- Finely mince intestinal tissue with sterile surgical scissors and incubate the tissue in Digestion Buffer A to strip off intestinal epithelial cells and intraepithelial lymphocytes (IELs) from the mucosal lining.
- Perform secondary digestion with Digestion Buffer B to degrade extracellular matrix components, yielding a heterogeneous cell mixture comprising lamina propria immune cells, fibroblasts, and smooth muscle cells.
- Conduct density gradient centrifugation using media such as BeyoColl™ to purify target lymphocytes and myeloid cells from the crude cell mixture.
Features
- This kit features a broad range of applications. Isolated immune cells can be used directly for various detection assays or further subjected to cell sorting to harvest specific cell subsets.
- The kit achieves a high yield of lamina propria immune cells. Specifically, immune cells isolated from the small intestinal lamina propria constitute over 80% of total viable cells, while those from the colonic lamina propria account for approximately 14% of viable cells. Immunomagnetic sorting with corresponding beads is recommended to obtain high-purity target cells for stringent downstream experiments.
Additional Notes
- This kit does not contain density gradient separation medium. It is recommended to use an isotonic density gradient medium with a homogeneous density ranging from 1.0–1.3 g/mL.
- After initial opening, Digestion Buffer A and Digestion Buffer B can be stored at 4 °C for 6 months. Repeated freeze–thaw cycles must be avoided.
- Prior to use, pre-warm Digestion Buffer A and Digestion Buffer B at 37 °C for 15–20 minutes.
- Supplement Reagent A (500X) is hazardous to humans. Wear appropriate protective equipment during operation to avoid direct skin contact and inhalation.
- For long-term cell culture applications, thoroughly flush intestinal luminal contents after tissue harvesting. If necessary, briefly incubate tissues with antibiotic-containing PBS to reduce bacterial contamination. After digestion, wash cells repeatedly with antibiotic-supplemented culture medium and filter through 70 μm or 40 μm cell strainers to remove tissue debris and bacterial aggregates.
- This product is for research use only. It is not intended for clinical diagnosis or treatment, food or pharmaceutical applications, nor for residential storage.
- For personal safety and health, always wear a lab coat and disposable gloves during experimentation.
Storage
Store the kit at −20 °C for a shelf life of 12 months. Digestion Buffer A, Digestion Buffer B and Digestion Stop Reagent (250X) can also be stored at 4 °C for up to 6 months.
Only for research and not intended for treatment of humans or animals
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