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Cat. No.: KLHAS-10 (for 10mg)

Cat. No.: KLHAS-100 (for 100mg)

Cat. No.: KLHAS-1k (for 1000mg)

Description

Shrimp Hemocyanin (SHC) is a highly soluble hemocyanin derived from shrimp. It can serve as an alternative to Keyhole Limpet Hemocyanin (KLH), functioning as a carrier protein for conjugation with peptides, nucleic acids, drugs, or toxins of low molecular weight, thereby conferring strong immunogenicity. Testing has shown that SHC possesses immunogenicity comparable to KLH, while its solubility—especially after conjugation with haptens—is significantly higher than KLH. This enhanced solubility provides greater flexibility in immunogen preparation strategies.

Product Form

Hemocyanin is supplied in lyophilized form, dissolved in PBS. For use, resuspend with ultrapure water.

Specifications

• Protein length: Full-length protein
• Animal-derived components: None
• Nature: Native
• Species: Penaeus vannamei
• Actual molecular weight: 75 kDa

Features

• High Immunogenicity – Proven to elicit strong immune responses.
• High Conjugation Yield – Efficient coupling with peptides, nucleic acids, drugs, or toxins.
• High Solubility – Significantly more soluble than KLH, even after hapten conjugation; compatible with poorly soluble antigens.
• High Purity – Greater than 98%.
• Outstanding Performance – Validated in hundreds of antibody preparation cases.
• High Cost-Effectiveness – Delivers superior value compared to KLH.

Hapten Conjugation with SHC

1. Preparation of SHC solution: Reconstitute lyophilized SHC (SMCC-activated) with ultrapure water to obtain a 10 mg/mL SHC solution.
2. Preparation of hapten solution: Dissolve 20 mg of thiol-containing hapten in 5 mL of conjugation buffer (83 mM sodium phosphate, 0.1 M EDTA, 0.9 M NaCl, 0.1 mM TCEP, pH 7.2). Note: 0.1 mM TCEP is optional. Its primary role is to reduce disulfide bonds in the hapten to free thiol groups.
3. Conjugation reaction: Immediately mix the hapten solution with activated SHC at a mass ratio of 1 : 0.6. Incubate at room temperature for 2 hours, or overnight at 4 °C. Note: This ratio is a recommended starting point. Hapten size and structural differences may affect conjugation efficiency. Multiple pilot experiments are advised to optimize the hapten-to-SHC ratio for best results.
4. Purification: Remove EDTA using size-exclusion chromatography.

Precautions

• Accurate Quantitation: Dissolve directly in the original vial to the desired concentration. Do not aliquot, as product properties and static charge may cause significant loss.
• Extinction Coefficient: The predicted extinction coefficient of SHC is ε1% = 10. Please adjust accordingly when measuring SHC solution concentration.
• Safety Precautions: For your safety and health, always wear a lab coat and disposable gloves during handling.
• Intended Use: This product is for research use only.

Related: Keyhole Limpet Hemocyanin (KLH) Alternative – Shrimp Hemocyanin (Unactivated)

Only for research and not intended for treatment of humans or animals

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