All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: HSTB-250 (for 250U)

Cat. No.: HSTB-1k (for 1000U)

For ligand-based hot-start DNA polymerase, please visit HM-Taq DNA Polymerase.

We also offer DNA-free HS-Taq DNA Polymerase, which has undergone a rigorous E. coli DNA removal process.

Description

HS-Taq DNA Polymerase (for bisulfite-treated DNA) is designed for PCR amplification of template DNA containing uracil residues after bisulfite treatment, ensuring exceptionally high amplification sensitivity and specificity.

In this formulation, Taq DNA polymerase and an anti-Taq antibody exhibit strong affinity. This interaction suppresses nonspecific amplification caused by primer mis-annealing or primer-dimer formation during sample preparation and the initial heating phase. The anti-Taq antibody is denatured during the initial DNA denaturation step of PCR, and therefore does not interfere with subsequent amplification.

Product Components

• HS-Taq (for bisulfite-treated DNA) (10 U/μL)
• 10X HS Taq Buffer (Mg²⁺ plus)

Unit Definition

One unit (U) is defined as the amount of enzyme required to incorporate 10 nmol of total deoxyribonucleotides into acid-insoluble material in 30 minutes at 74 °C, using salmon sperm DNA as template/primer.

Quality Control

• Free of detectable endonuclease and exonuclease contamination.
• Free of RNase contamination.
• Residual E. coli genomic DNA ≤ 10 copies per 10 U (as determined by qPCR).
• Functional assay: In a 25 μL PCR system containing 0.5 U of this enzyme, bisulfite-treated SiHa cell DNA is used as template. After 35 cycles, electrophoresis of the PCR product on a 1.5% agarose gel shows a single, specific target band.

Storage

Store at -20°C, with a minimum shelf life of 2 years.

SBS Genetech is recognized as one of the global major leading industry players in DNA Polymerase by third-party market researchers. For more details, please visit DNA Polymerase Market Size To Reach USD 568.3 Million In 2030 | Rising Demand For Customized DNA Polymerase And Next-Generation DNA Sequencing Are Some Of The Key Factors Driving Market Revenue Growth, Says Reports and Data

Only for research and not intended for treatment of humans or animals

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory