The difference between the HM-Taq DNA Polymerase and the general hot start Taq DNA Polymerase is that the general hot start Taq DNA Polymerase only blocks the polymerase activity before the temperature rise in the first step, while the HM-Taq DNA Polymerase uses the inhibitory ligand to block the substrate-binding site of the HM-Taq DNA Polymerase through temperature regulation. When the temperature is lower than 40°C, an inactive enzyme-inhibitor complex is formed. When the temperature rises to the annealing temperature, the binding balance moves towards the formation of the template-primer complex, so the production of non-specific amplification products in the whole process of PCR amplification is minimized and the accuracy of PCR reaction is greatly improved. The 3' end of PCR product is A, which can be cloned directly with the TA vector.
Fast process: no additional heating is required for activation.
Better control: The enzyme activity is controlled during continuous annealing to achieve the whole process hot start effect.
Minimal contamination: There is no protein contamination such as denatured antibodies in the PCR process.
One active unit is defined as the amount of enzyme required to add 10 nmol deoxynucleotides into acid-insoluble substances with activated salmon sperm DNA as template primer at 74°C and 30 minutes.
The purity of SDS-PAGE is more than 99%, and there is no exogenous nuclease activity
Free of host residual DNA by PCR detection
Effectively amplify single-copy genes in the human genome
There is no significant change in activity after storage at room temperature for one week.
It is generally used for genome amplification with high sensitivity and strong background (such as the detection of a specific gene site in the genome or exogenous pathogens), DNA sequencing, multiplex PCR, TA cloning, etc.