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Cat. No.: FPVPQ-50 (for 50T)

Cat. No.: FPVPQ-200 (for 200T)

Description

Feline Panleukopenia Virus (FPV) Probe qPCR Kit is a quick, efficient, and highly sensitive kit for detecting feline parvovirus infections using quantitative PCR (qPCR) with a probe method. This anti-contamination product contains an optimized ratio of high-quality UDG enzyme and dUTP, which effectively eliminates false positives or low CT values caused by product contamination during PCR amplification.

Feline Panleukopenia Virus (FPV), also known as feline distemper virus or feline infectious enteritis virus, is a single-stranded DNA virus belonging to the Parvoviridae family. Under an electron microscope, FPV appears round or hexagonal without an envelope. The genome is approximately 5200 nucleotides long and contains two open reading frames (ORFs) that encode structural proteins (VP1 and VP2) and non-structural proteins (NS1 and NS2). Infected animals experience a sharp decrease in white blood cells, severe fever, vomiting, and diarrhea. The virus primarily affects young cats and is highly contagious among all felines, with a high mortality rate. It spreads rapidly through the feces, urine, and vomit of infected animals and can be vertically transmitted during pregnancy, leading to cerebellar hypoplasia in fetuses. Early and rapid diagnosis of FPV is crucial to isolate infected cats and provide targeted treatment to reduce morbidity and mortality.

The main symptoms of FPV infection are fever, vomiting, and diarrhea, which can easily be confused with other gastrointestinal diseases clinically, making diagnosis based solely on clinical symptoms difficult. Current clinical diagnostic methods for FPV infection include electron microscopy (EM), virus isolation (VI), latex agglutination test (LAT), hemagglutination (HA), ELISA, and PCR analysis. Although electron microscopy and virus isolation are specific and sensitive, they are expensive and time-consuming in clinical use. Latex agglutination is fast but lacks specificity. Hemagglutination or serum hemagglutination inhibition tests can produce false negatives due to the weak hemagglutination activity of FPV and stringent operating conditions. While ELISA and PCR are widely used for their high sensitivity and specificity, they are somewhat complex to operate. qPCR, however, is straightforward and does not require electrophoresis after PCR amplification to confirm FPV infection.

This kit provides the necessary components for qPCR experiments, including the pre-mixed Probe qPCR Mix (2X) and specific primer-probe mixture. The pre-mix contains Fast Taq DNA Polymerase, UDG enzyme, PCR Buffer, dNTPs, dUTP, stabilizers, and magnesium ions. The primer-probe mixture targets the most conserved sequence of the FPV VP2 gene, enabling ultra-sensitive quantitative detection of FPV DNA. Additionally, the kit includes a Positive Control to verify the proper functioning of the kit itself.

This kit provides both Low ROX and High ROX, making it widely compatible with fluorescent quantitative PCR instruments that either do not require ROX or need Low ROX or High ROX as a reference dye. ROX is used to correct for fluorescence fluctuations that are unrelated to PCR, thus minimizing well-to-well variation. This variation can be caused by factors such as pipetting errors and sample evaporation. Different fluorescent quantitative PCR instruments have varying ROX requirements; please choose the appropriate concentration of ROX (High ROX, Low ROX, or no ROX) based on the instrument used. Refer to the table below for the ROX type required by commonly used instruments.

Components

• Probe qPCR Mix (2X)
• Primer/Probe Mix
• Positive Control
• Low ROX (50X)
• High ROX (50X)
• Nuclease-free Water