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Cat. No.: ECLSM-100 (for 100ml)

Cat. No.: ECLSM-500 (for 500ml)

Description

ECL SuperMoon is an ultra-sensitive chemiluminescence reagent kit based on luminol. It offers significantly better luminescence performance compared to ECL Moon. It reacts chemically with horseradish peroxidase (HRP) conjugated to the secondary antibody, emitting fluorescence. This fluorescence can be detected using suitable chemiluminescence imaging equipment or X-ray film.

We offer four types of Western chemiluminescent detection reagents: ECL Plus, ECL Star, ECL Moon, and ECL SuperMoon. For routine Western detection, we recommend using ECL Star. For detecting proteins with relatively high abundance, such as internal reference proteins, the more cost-effective ECL Plus can also be used. For low-abundance and difficult-to-detect target proteins, we recommend using the highly sensitive ECL Moon or the even more sensitive ECL SuperMoon. However, for detecting proteins with moderate abundance, we do not recommend using ECL Moon or ECL SuperMoon, as they are prone to causing overexposure

Performance

ECL SuperMoon has extremely high sensitivity, with Western blot detection results significantly better than ECL Moon. The detection sensitivity can be approximately doubled or more compared to ECL Moon.

ECL SuperMoon's high sensitivity significantly conserves precious samples as well as expensive primary and secondary antibodies. It is important to appropriately reduce the amount of primary and secondary antibodies or samples to avoid producing overly intense bands that are difficult to compare.

ECL SuperMoon has a very low background and is suitable for both PVDF and nitrocellulose membranes. PVDF membranes and nitrocellulose membranes can be ordered from us.

Components

• ECL SuperMoon Solutions A
• ECL SuperMoon Solutions B

Storage Conditions

Store at 4°C in the dark for one year. If stored at 37°C in the dark, it is effective for one month. For long-term storage, keep at -20°C in the dark. At -20°C, it can be preserved for a longer period.

Notes

• Solutions A and B of ECL SuperMoon must have their pipette tips changed during use. Contamination between Solutions A and B can cause them to gradually lose efficacy and affect subsequent performance.
• After using each solution, ensure that the bottle caps are tightly closed to prevent degradation. This is particularly important for Solution B, which contains oxidizers and is prone to reduction and loss of effectiveness.
• ECL SuperMoon has a long fluorescence duration, but the fluorescence is stronger during the first 20-30 minutes after the reaction starts. The fluorescence will gradually decrease thereafter, so take full advantage of this stronger fluorescence period for imaging.
• If you find that ECL SuperMoon's detection sensitivity is too high and affects the results, you can opt for a less sensitive reagent such as ECL Moon or ECL Star, or reduce the amount of sample, primary antibody, or secondary antibody used.
• Both Solutions A and B are harmful to humans. Handle with care and use appropriate protection to avoid direct contact with skin or inhalation.
• This product is for scientific research use by professionals only and is not intended for clinical diagnosis or treatment, food or drug use, and should not be stored in residential areas.
• For your safety and health, please wear a lab coat and disposable gloves while handling the product.

Related:

• ECL Plus Western Blotting Substrate
• ECL Star Western Blotting Substrate
• ECL Moon Western Blotting Substrate
• ECL SuperMoon Western Blotting Substrate