All products have special prices for bulk purchase, please contact us for more details if required.

Cat. No.: CGRC-500 (for 50-500T)

Cat. No.: CGRC-2k (for 200-2000T)

Description

Cell Tracking Red Fluorescence Staining Kit with Red CMTPX is a kit for live cell fluorescent tracking using Red CMTPX as the red fluorescent probe. This kit is compatible with fluorescence detection systems including fluorescence microscopes, laser scanning confocal microscopes, fluorescence microplate readers, and flow cytometers. The kit supports subsequent cell fixation and permeabilization, and it is also suitable for staining dead cells or pre-fixed cells.

Red CMTPX has a molecular formula of C₄₂H₄₀ClN₃O₄, a molecular weight of 686.24, and a CAS number of 942416-35-5. As a cell-membrane-permeable fluorescent probe, Cell-Tracker™ Red CMTPX penetrates the cell membrane and enters live cells. The chloromethyl group on the probe covalently binds to intracellular proteins, forming a membrane-impermeable reaction product with red fluorescence.

This product features the following properties:

• It emits bright red fluorescence upon excitation, with an excitation maximum at approximately 592 nm and an emission maximum at approximately 611 nm.
• Its chloromethyl group reacts irreversibly with intracellular glutathione (GSH) catalyzed by glutathione S-transferase (GST) to form a fluorescent product that is well retained within cells, enabling long-term labeling.
• The fluorescent signal remains highly stable at physiological pH for at least 72 hours, making it ideal for tracking studies of cell motility, localization, proliferation, migration, chemotaxis and invasion.
• In general, the fluorescent stain is passed only to daughter cells and does not transfer to neighboring cells.
• It is non-toxic and does not affect cell viability or proliferation.

Storage

Store at -20 ºC; valid for 1 year. Red CMTPX (1000X) must be protected from light.

Precautions

• Repeated freeze-thaw cycles of Red CMTPX (1000X) will compromise detection performance. Aliquot appropriately upon first use to avoid repeated freeze-thawing.
• For trace volumes of liquid, centrifuge for several seconds before each use to fully collect liquid at the bottom of the tube.
• Fluorescent dyes are susceptible to photobleaching. Minimize light exposure to slow down fluorescence quenching.
• This product is for research use only by professionals. It shall not be used for clinical diagnosis or therapy, food or pharmaceutical applications, and shall not be stored in residential premises.
• For your safety and health, wear a lab coat and disposable gloves during handling.