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Cat. No.: CGGC-500 (for 50-500T)

Cat. No.: CGGC-2k (for 200-2000T)

Description

Cell Tracking Green Fluorescence Staining Kit with Green CMFDA is a kit for live cell fluorescent tracking using Green CMFDA as the green fluorescent probe. This kit is compatible with fluorescence detection systems including fluorescence microscopes, laser scanning confocal microscopes, fluorescence microplate readers, and flow cytometers. The kit supports subsequent cell fixation and permeabilization; however, fluorescence intensity will decrease to a certain extent if fixation and permeabilization are performed after staining. This kit cannot be used for staining dead cells or pre-fixed cells.

Green CMFDA, full name 5-Chloromethylfluorescein Diacetate, is a chloromethyl derivative of fluorescein diacetate (FDA). Its molecular formula is C₂₅H₁₇ClO₇, molecular weight 464.85, and CAS number 136832-63-8. As a cell-membrane-permeable fluorescent probe, Cell-Tracker™ Green CMFDA penetrates the cell membrane and enters live cells. Once inside the cytoplasm, it is converted into the membrane-impermeable reaction product 5-Chloromethylfluorescein (CMF).

This product features the following properties:

• It emits bright green fluorescence upon excitation, with an excitation maximum at approximately 492 nm and an emission maximum at approximately 516 nm.
• Its chloromethyl group reacts irreversibly with intracellular glutathione (GSH) catalyzed by glutathione S-transferase (GST) to form a fluorescent product that is well retained within cells, enabling long-term labeling.
• The fluorescent signal remains highly stable at physiological pH for at least 72 hours, making it ideal for tracking studies of cell motility, localization, proliferation, migration, chemotaxis and invasion.
• Within a cell population, the dye is passed only to daughter cells and does not transfer to neighboring cells.
• It is non-toxic and does not affect cell viability or proliferation.

Storage

Store at -20 ºC; valid for 1 year. Green CMFDA (1000X) must be protected from light.

Precautions

• Repeated freeze-thaw cycles of Green CMFDA (1000X) will compromise detection performance. Aliquot appropriately upon first use to avoid repeated freeze-thawing.
• For trace volumes of liquid, centrifuge for several seconds before each use to fully collect liquid at the bottom of the tube.
• Fluorescent dyes are susceptible to photobleaching. Minimize light exposure to slow down fluorescence quenching.
• This product is for research use only by professionals. It shall not be used for clinical diagnosis or therapy, food or pharmaceutical applications, and shall not be stored in residential premises.
• For your safety and health, wear a lab coat and disposable gloves during handling.