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Cat. No.: CPCTK-200 (for >200T)

Description

Calcium Phosphate Cell Transfection Kit is an improvement upon traditional calcium phosphate cell transfection methods, enhancing transfection efficiency while reducing toxicity. Transfecting cells using the calcium phosphate method allows for both transient expression and the selection of stable clones.

HEK293 or HEK293T cells, commonly referred to as 293 cells, are among the most suitable cells for calcium phosphate transfection. With optimized conditions, transfection efficiency can exceed 90%, and typically, it is easily achieved around 40-50%. Most common cell lines such as HeLa cells, CHO cells, etc., are also suitable for calcium phosphate transfection, although the efficiency may be slightly lower than that of 293 cells.

This kit is not only suitable for transfecting most adherent cells but also applicable for transfecting some suspension cells.

All reagents provided in this kit are sterile and can be used directly.

The kit is sufficient to transfect no fewer than 200 cell samples.

Components

• BBS solution
• Calcium chloride solution

Storage

Store at -20°C and valid for one year.

Precaution

• During transfection, add the DNA-calcium chloride solution to the BBS solution; do not add the BBS solution to the DNA-calcium chloride solution.
• High-purity plasmids are essential for achieving high transfection efficiency. Ensure that the A260/A280 ratio is greater than 1.8, and verify through gel electrophoresis that over 90% of the extracted plasmids exhibit supercoiling.
• When using the calcium phosphate method for cell transfection, maintain a carbon dioxide concentration not exceeding 5%, with an optimal concentration around 3%.
• The pH of the BBS solution directly impacts transfection efficiency. Avoid prolonged exposure of the BBS solution to air to prevent carbonation by atmospheric carbon dioxide.
• Due to variations in plasmids and cells, the optimal plasmid amount for transfection needs to be determined empirically.
• This product is intended for scientific research by professionals only and should not be used for clinical diagnosis or treatment, food, or drugs, nor stored in residential homes.
• For your safety and health, wear laboratory attire and disposable gloves during operation.

Transfection Reagent Portfolio

• TransEZPEI™ Transfection Reagent
• TransEZ293F™ Transfection Reagent
• TransEZ293™ Transfection Reagent
• TransEZ6000™ Transfection Reagent
• TransEZ8000™ Transfection Reagent
• TransEZRNAi™ Transfection Reagent
• TransEZInsect™ Transfection Reagent

SBS Genetech is recognized as one of the global major leading industry players in Transfection Reagent by third-party market researchers. For more details, please visit Global Transfection Reagent and Equipment Market – Industry Trends and Forecast to 2030.

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory