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Cat. No.: BTPR-50 (for 50T)

Cat. No.: BTPR-200 (for 200T)

Description

Bone tissue is hard with a low bone cell density. Furthermore, the peripheral matrix contains a large amount of mucoprotein (proteoglycan) which makes RNA difficult to separate, rendering traditional Trizol methods unsuitable for high-quality extraction. This kit employs a unique lysis solution that does not contain phenol/chloroform and incorporates various components to eliminate the proteoglycans in bone tissue. The proprietary genomic DNA removal column technology effectively clears gDNA residues, and the resulting RNA typically does not require DNase digestion, making it suitable for reverse transcription PCR, fluorescence quantitative PCR, and other experiments. The special lysis solution swiftly breaks down cells and inactivates cellular RNA enzymes. Centrifugation is used to precipitate and remove polysaccharides and secondary metabolites. The lysed mixture is then conditioned with ethanol, allowing RNA to bind and adsorb to the genomic DNA removal column. The RNA is selectively eluted and filtered, while the residual DNA adsorbed on the genomic DNA removal column cannot be eluted and is discarded with the column, thereby removing the DNA. The filtered RNA, after conditioning with ethanol, selectively adheres to the silica matrix membrane inside the centrifuge column under high salt conditions. Through a series of rapid wash-centrifugation steps, the protein removal liquid and wash solution eliminate cellular metabolites, proteins, and other impurities. Finally, low salt RNase-free H2O elutes the pure RNA from the silica matrix membrane.

Features

• The silica matrix membrane inside the centrifugal adsorption column is exclusively sourced from a world-renowned company, ensuring minimal adsorption differences between columns and good repeatability. This overcomes the inconsistency in membrane quality found in common kits.
• There is no need to use toxic reagents like phenol and chloroform, nor is there a requirement for steps like ethanol precipitation.
• The process is rapid and straightforward; the operation for a single sample can generally be completed within 30 minutes.
• Multiple column washes ensure high purity. The typical OD260/OD280 ratio reaches 1.9 to 2.0, with virtually no DNA residues remaining. The purified product is suitable for RT-PCR, Northern-blot, and various other experiments.

Storage

This kit can be stored at room temperature for 12 months without affecting its performance.