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Cat. No.: BITMB-1 (for 1ml)

Cat. No.: BITMB-5 (for 5ml)

Description

Biotin Magnetic Beads, also referred to as Biotin Beads, are composed of high-quality biotin covalently coupled with superparamagnetic nanometer-scale beads. These beads enable rapid, efficient, sensitive, and specific binding to streptavidin or avidin, as well as to antibodies, nucleic acids, proteins, peptides, lectins, or other molecules conjugated with streptavidin or avidin. They are primarily used for the separation and purification of nucleic acids, antibodies, proteins, or related complexes that are conjugated with streptavidin or avidin. Applications include immunoprecipitation, cell sorting, and the study of DNA-protein interactions.

Biotin, also known as Vitamin B7, D-Biotin, Vitamin H, Biso II, or Coenzyme R, is one of the B vitamins. It is classified as a heterocyclic compound, containing a sulfur-containing ring fused with a ureido group (-N-CO-N-) and a tetrahydrothiophene group. The ureido ring acts as a carbon dioxide carrier during carboxylation reactions. Biotin plays a role in carbohydrate digestion, fatty acid synthesis, and gluconeogenesis. Its biotinylation in histones within nucleochromatin contributes to chromatin stability and gene expression regulation. Additionally, biotin functions as a cofactor for carboxylases, such as pyruvate carboxylase, which catalyzes the formation of oxaloacetate from pyruvate and carbon dioxide.

Biotin is a small molecule that does not interfere with the biological function of larger molecules. It is widely utilized in the biotin-(strept)avidin system, serving as an important cofactor in various metabolic pathways. The strong affinity between biotin and streptavidin/avidin facilitates the conjugation of target molecules (e.g., antibodies, nucleotides, or proteins) with labeling systems, such as enzymes, fluorescent, or chemiluminescent probes. These complexes are applied in numerous detection systems, including immunoprecipitation, immunohistochemistry/flow cytometry, and Southern and Northern blotting. Furthermore, this method is used for the purification and characterization of various target molecules. Biotin also finds applications in cultivating oligodendrocytes, as a vitamin supplement for Bacillus species growth, and in blocking endogenous biotin during immunohistological procedures.

Biotin Magnetic Beads are extensively employed in the biomedical field. They specifically bind to streptavidin/avidin-conjugated antigens or antibodies, serving as carriers in applications such as immunoprecipitation, cell sorting, and ELISA. They can also bind to streptavidin/avidin-conjugated DNA or RNA fragments to isolate specific nucleic acid-protein complexes from cell or tissue extracts, facilitating studies on protein-nucleic acid interactions. Additionally, these beads can bind to streptavidin/avidin-conjugated nucleic acid probes for use in DNA/RNA hybridization experiments or the isolation and purification of mRNA. Other applications include purifying single-stranded DNA oligonucleotides conjugated with streptavidin/avidin and separating streptavidin/avidin-conjugated PCR products.

Features

• Exceptional Binding Capacity This product offers an outstanding binding capacity compared to many similar products. It enables rapid and efficient separation and purification of streptavidin-conjugated molecules in complex samples, with minimal nonspecific adsorption due to its small bead size. Each milliliter of bead suspension contains approximately 10 mg of magnetic beads with high-quality biotin, capable of binding ≥30 μg of streptavidin per milligram of beads, and up to 60 μg in optimal conditions. This high efficiency makes it ideal for immunoprecipitation experiments and other applications.
• Strong Specificity The product exhibits strong specificity for streptavidin-conjugated ligands, including antibodies, nucleic acids, proteins, peptides, and lectins. The high purity of the isolated products makes them suitable for subsequent analysis, such as Western blotting, ELISA, Northern blotting, qPCR, and mass spectrometry.
• Rapid Binding Designed for speed and efficiency, the product facilitates fast ligand binding due to its nanometer-scale beads (~200 nm) with an exceptionally large surface area. This reduces the time required for streptavidin and biotin interaction, preserving the activity and integrity of the ligands by avoiding degradation during prolonged handling processes.
• Ease of Use Stored in a specially formulated protective solution without glycerol, the product is easy to use with magnetic separation methods, eliminating the need for centrifugation. It is compatible with both small-scale sample detection and high-throughput screening in automated systems, ensuring consistent results across different operational methods.

Parameters

Precautions

• This product should be maintained at a pH of 6-8 and avoid high-speed centrifugation or drying. Do not leave magnetic beads in the magnetic field for extended periods, as this may cause bead aggregation.
• Before using this product, resuspend it adequately by inverting several times to ensure the beads are evenly mixed. Mixing should be done gently, avoiding vigorous vortexing or shaking to prevent protein denaturation.
• When performing immunoprecipitation or purification, it is recommended to design positive and negative control groups. The type, size, and coupling method and extent (streptavidin-affinity) of the target molecules will affect binding efficiency. It is suggested to determine the bead usage for each specific application through titration methods, and consider increasing the bead amount to 2-3 times the molar quantity of the target molecules to ensure sufficient binding.
• Protein samples should be purified promptly after collection and always kept at 4ºC or in an ice bath to slow protein degradation or denaturation. To effectively inhibit protein degradation, an appropriate amount of protease inhibitor mixture can be added to the protein sample.
• If using a vacuum pump or similar instrument to aspirate the supernatant, pay attention to the suction strength to avoid drawing up aggregated beads due to excessive suction. Beads may aggregate during acidic solution elution, which is normal and does not affect their proper use. A 0.1% solution of non-ionic detergents (such as Triton X-100, Tween-20, or NP-40) can effectively prevent bead aggregation without affecting antibody binding efficiency.
• Beads cannot be reused after acidic solution elution or SDS-PAGE elution. To minimize biotin loss, whether manual or automated, low pH elution steps should not exceed 10 minutes.
• High concentrations of DTT, beta-mercaptoethanol, or guanidine hydrochloride may affect the binding of this product to ligands.
• This product is for scientific research use only by professionals; it is not intended for clinical diagnosis or treatment, food, or drug use, and should not be stored in a residential setting.
• For your safety and health, please wear lab coats and disposable gloves during operation.

Storage 

Store at 4℃ for up to one year. For extended storage, especially if not in use for a long time, it is recommended to store at -20℃ to ensure a longer shelf life.

Only for research and not intended for treatment of humans or animals

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