Cat. No.: APQL-200 (for 200Tx20μl)

Cat. No.: APQL-1k (for 1000Tx20μl)

Description

The core enzyme of Aptamer Probe qPCR LyoMix is assembled with HotStart Aptamer Taq DNA Polymerase, specifically designed for TaqMan probe-based qPCR amplification of DNA templates.

Content

• 2.5 x Aptamer qPCR LyoMix

Features

• HotStart Aptamer Taq DNA Polymerase features a high-affinity nucleic acid aptamer (Aptamer) that blocks the enzyme's active site. Consequently, Taq DNA Polymerase remains inactive at low temperatures. As the temperature increases to 50°C, the nucleic acid aptamer dissociates from the enzyme's active site, thereby releasing its activity. The Aptamer possesses full-length amplification control capability, facilitating high sensitivity amplification and multiple TaqMan PCR amplifications. Moreover, HotStart Aptamer Taq DNA Polymerase is a rapid enzyme with an extension rate of 4kb/min. In fast qPCR protocols, amplification and probe cleavage can be achieved within 10 seconds of annealing and extension.

• The exclusive lyophilization formula of this product endows it with versatility, meeting not only routine research needs but also enabling simultaneous development and production of lyophilized products under one reagent. Using this reagent eliminates the need for adding any other auxiliary reagents to complete the lyophilized product system.

Storage

For long-term storage, please store at -20°C or below (effective for 18 months). The product remains unaffected after 10 repeated freeze-thaw cycles, but repeated freeze-thawing should be avoided. For short-term storage, it is recommended to store at 2-8°C, where the product remains stable for 2 weeks. Due to the high concentration of lyophilized excipients, the product may contain white insoluble material upon reconstitution. In this case, the product can be completely dissolved by placing it at 30°C (or in the palm of the hand) for 3-5 minutes, without any degradation in performance.

Related:

• HotStart Aptamer Taq DNA Polymerase
• Aptamer Probe qPCR LyoMix
• Aptamer Probe exRT-qPCR LyoMix

Only for research and not intended for treatment of humans or animals

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory