Anti-Flag Affinity Gel
$199.00 - $1,999.00
All products have special prices for bulk purchase, please contact for more details if required.
Cat. No.: AFAG-0.5 (for 0.5ml)
Cat. No.: AFAG-2 (for 2ml)
Cat. No.: AFAG-10 (for 10ml)
Anti-Flag Affinity Gel, also known as Anti-Flag IP Gel, Anti-Flag immunoprecipitation gel, or Anti-Flag agarose gel, is formed by covalently coupling high-quality monoclonal mouse-derived Flag antibodies to agarose. It can specifically bind to proteins containing the Flag tag in common protein expression systems such as mammalian cells, bacteria, or yeast, enabling immunoprecipitation (IP) or purification of fusion proteins or protein complexes with Flag tags.
Flag tag, Myc tag, HA tag, His tag, and GST tag are some of the most common tags found on expression vectors. Fusion expression with these tags facilitates convenient detection of the target protein and proteins that interact with the target protein. It also allows for easy purification of the target protein. The Flag tag is an octapeptide composed of eight amino acid residues (DYKDDDDK), commonly represented as Flag or 3X Flag. The nucleotide sequence encoding the Flag tag is genetically fused to the 5' or 3' end of the target gene using recombinant techniques, resulting in the expression of the target protein with the Flag tag. The Flag tag offers the following advantages: it generally does not interact with the target protein and, in most cases, does not affect the function of the target protein. As a tagged protein, the Flag tag allows for subsequent detection, localization, and functional analysis of the target gene using Flag antibodies, Anti-Flag magnetic beads, or Anti-Flag affinity gels. Fusion proteins with the Flag tag at the N-terminus can be cleaved specifically by enterokinase (EK) after the Flag sequence (DDDK), resulting in the production of the target protein without the tag. Based on these advantages, the Flag tag has been widely used in various aspects of protein expression, purification, identification, protein-protein interactions, and functional studies.
Anti-Flag Affinity Gel, also known as Anti-DYKDDDDK Affinity Gel/Beads/Resin or Anti-DYKDDDDK IP Gel/Beads/Resin, can specifically bind to fusion proteins with the Flag tag. It is widely used in immunoprecipitation or purification of fusion proteins or protein complexes with the Flag tag.
Only for research and not intended for treatment of humans or animals
SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory