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Cat. No.: ARDMP-50 (for 50T)

Cat. No.: ARDMP-200 (for 200T)

Description

This kit is designed for the rapid extraction and separation of genomic DNA, total RNA including miRNA, and Protein from the same animal cell or tissue sample. If you purchase additional miRNA adsorption columns and matching solutions, you can also separate and extract total RNA and miRNA from the same sample, enriching miRNA. The unique lysis solution quickly lysates cells and inactivates cellular RNA/DNA enzymes. Then, the lysis mixture RNA/miRNA/DNA/Protein simultaneously passes through a genomic DNA adsorption column. Genomic DNA is adsorbed, while miRNA/RNA/Protein passes through. After a series of wash-centrifugation steps on the DNA adsorption column, pure genomic DNA is eluted. The miRNA/RNA filtrate is adjusted with ethanol to determine binding conditions, and miRNA/RNA selectively adheres to the miRNA/RNA adsorption column under high salt conditions. After a series of quick wash-centrifugation steps, pure miRNA/RNA is eluted. The filtrate undergoes selective precipitation to obtain Protein. Based on the phenol-free and chloroform-free DNA/RNA rapid extraction technology combined with exclusive separation technology, the obtained miRNA/RNA/genomic DNA is of high purity and does not interfere with each other. The resulting miRNA/RNA does not need DNase digestion and can be directly used for reverse transcription PCR, fluorescent quantitative PCR, and other experiments. The genomic DNA can also be directly used for downstream experiments such as Southern blotting, enzyme digestion, PCR, and more.

Features

• Does not use toxic chemicals like phenol, chloroform, etc., and there's no need for ethanol precipitation steps.
• Fast and straightforward, the separation process of miRNA/RNA/genomic DNA/Protein from a single sample can generally be completed within 1 hour.
• The exclusive adsorption column and formula ensure effective removal of genomic DNA residues. In most cases, the obtained miRNA/RNA does not require DNase digestion and can be used directly for reverse transcription PCR, quantitative fluorescence PCR, and other experiments.
• Multiple column washes ensure high purity of miRNA/RNA/genomic DNA, which can be directly used for various downstream experiments.

Storage

This kit can be stored at room temperature for 12 months without affecting its performance.