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Cat. No.: T2PMD-5 (for 5 x 1ml)

Cat. No.: T2PMD-100 (for 100 x 1ml)

Description

The concentration of Taq 2.0 PCR Mix (+Dye) is 2×, making it convenient and quick to use, reducing contamination during the PCR process. To use, simply take an appropriate amount of 2× Taq 2.0 PCR Mix (+Dye), add the template and primers, and add ddH₂O to make the reaction system concentration 1×, then proceed with the PCR reaction. The PCR product has an overhanging A base at the 3’ end, which can be directly used for T/A cloning after purification.

The Taq DNA polymerase in this mix is a mutant of Taq 2.0 DNA Polymerase, which can efficiently amplify DNA fragments ≤5 kb, has good inhibitor tolerance, and its amplification speed is about three times that of ordinary Taq DNA polymerase. This significantly reduces the time required for the PCR extension process, thereby shortening the entire PCR reaction time. Unlike fusion protein-based fast Taq polymerases, Taq 2.0 is more similar to WT-Taq, minimizing issues such as band smearing, trailing, or alteration of fragment sizes.

This PCR Mix contains two dyes, so the PCR product can be directly loaded for electrophoresis without adding Loading Buffer. During electrophoresis, two indicator bands, purple and yellow, will appear. These dyes do not affect PCR amplification efficiency, but for experiments requiring optical analysis of PCR products, such as absorbance or fluorescence, it is recommended to purify the PCR products before analysis.

Nuclease Activity Detection

Mix 25 μl of 2× Taq 2.0 PCR Mix (+Dye) with 200 ng of supercoiled plasmid DNA to form a 50 μl reaction system. Incubate together at 37°C for 4 hours, then use agarose gel electrophoresis to detect that less than 10% of the plasmid DNA has converted to nicked or linear forms.

Non-specific Nuclease Activity Detection

Mix 25 μl of 2× Taq 2.0 PCR Mix (+Dye) with 15 ng of double-stranded DNA fragments to form a 50 μl reaction system. Incubate at 37°C for 16 hours, then use agarose gel electrophoresis to detect that the double-stranded DNA substrate remains unchanged.

Storage

The minimum shelf life is 1 year at -20°C.

Related: U-Taq DNA Polymerase

Only for research and not intended for treatment of humans or animals

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