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miRNA
siRNA
The common methods of mammalian transfection include:
Currently, liposome transfection is most commonly used.
Several aspects need to be paid attention to for liposome transfection:
The most difficult problem of RNAi technology is the stability of siRNA. Here, with chemical modification, our chemically modified siRNA has much higher stability than the common siRNA. The chemical modification not only enhances the life span of siRNA in serum and cell culture but also enhances its activity in vitro.
Sirnafectamine is a new generation of the RNA transfection reagent, which can be used in RNA transfection experiments. Sirnafectamine accelerates the binding of glycoprotein on cell membrane, which promotes the endocytosis of cells. In addition, compared with other siRNA transfection reagents, Sirnafectamine has minimal cytotoxicity and the best cell state after transfection.
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