from China, for the World
for Superior Biology Services since 2000
- Product
- All Products
- Custom Services
- Catalog Products
- Innovative Systems
- Nucleic Acid Related
- Natural Compounds
- Synthetic Biology
- Enzymes
- POCT Solution
- LAMP
- RPA
- CRISPR
- Freeze-Drying System
- Lateral Flow System
- About
- About SBS
- Achievements
- Ecosystem
- Legal Statement
- …
- Product
- All Products
- Custom Services
- Catalog Products
- Innovative Systems
- Nucleic Acid Related
- Natural Compounds
- Synthetic Biology
- Enzymes
- POCT Solution
- LAMP
- RPA
- CRISPR
- Freeze-Drying System
- Lateral Flow System
- About
- About SBS
- Achievements
- Ecosystem
- Legal Statement
from China, for the World
for Superior Biology Services since 2000
- Product
- All Products
- Custom Services
- Catalog Products
- Innovative Systems
- Nucleic Acid Related
- Natural Compounds
- Synthetic Biology
- Enzymes
- POCT Solution
- LAMP
- RPA
- CRISPR
- Freeze-Drying System
- Lateral Flow System
- About
- About SBS
- Achievements
- Ecosystem
- Legal Statement
- …
- Product
- All Products
- Custom Services
- Catalog Products
- Innovative Systems
- Nucleic Acid Related
- Natural Compounds
- Synthetic Biology
- Enzymes
- POCT Solution
- LAMP
- RPA
- CRISPR
- Freeze-Drying System
- Lateral Flow System
- About
- About SBS
- Achievements
- Ecosystem
- Legal Statement
RNA Silencing
At SBS Genetech, we are at the forefront of offering high-quality siRNA and transfection reagents, facilitating the readiness of successful RNA silencing.
What is RNA Silencing?
RNA Silencing is a conserved RNA-mediated and sequence-specific gene silencing effect by which gene expression is down-regulated by either microRNA (miRNA) or small interfering RNA (siRNA)
miRNA
- A small single-stranded non-coding RNA molecule
- Endogenously expressed
- Inhibit translation of multiple mRNA targets
- 19-25 nucleotides
siRNA
- A small double-stranded non-coding RNA molecule
- Exogenously derived
- Highly specific with only one mRNA target
- 21-23 nucleotides
RNA Silencing Mechanism
- dsRNA is first degraded by Dicer (a highly conserved RNase) into siRNA (~22 bp).
- siRNA binds to RISC (RNA Induced Silencing Complex) to form siRNA-Protein Complex.
- The Complex cleaves mRNA with a complementary sequence, inhibiting downstream expression.
A Successful RNA Silencing Expriment
Transfection Methods
The common methods of mammalian transfection include:
- calcium phosphate coprecipitation
- electroporation
- microinjection
- gene gun
- liposome transfection
- etc
Currently, liposome transfection is most commonly used.
Notes for Liposome Transfection
Several aspects need to be paid attention to for liposome transfection:
- the type and dosage of transfection reagent
- the type and dosage of siRNA
- the cell density
- the operation sequence
- the water bath timing of cells and siRNA-transfection reagent complex
Chemically Modified siRNA
The most difficult problem of RNAi technology is the stability of siRNA. Here, with chemical modification, our chemically modified siRNA has much higher stability than the common siRNA. The chemical modification not only enhances the life span of siRNA in serum and cell culture but also enhances its activity in vitro.
Sirnafectamine Transfection Reagent
Sirnafectamine is a new generation of the RNA transfection reagent, which can be used in RNA transfection experiments. Sirnafectamine accelerates the binding of glycoprotein on cell membrane, which promotes the endocytosis of cells. In addition, compared with other siRNA transfection reagents, Sirnafectamine has minimal cytotoxicity and the best cell state after transfection.
RNA Silencing Products
Buy nowmiRNA Antagomir$850.00Buy nowmiRNA Agomir$850.00Buy nowChemically Modified miRNA Inhibitor$245.00 - $350.00$500.00Buy nowChemically Modified miRNA Mimic$245.00 - $350.00$500.00Buy nowChemically Modified siRNA$210.00 - $2,275.00$3,250.00Buy nowCommon siRNA$122.50 - $2,100.00$3,000.00Buy nowRNAi Control$42.00 - $56.00$80.00Buy nowSirnafectamine Transfection Reagent$56.00 - $525.00$750.00Buy nowFluorescently Labeled siRNA$280.00 - $3,150.00$4,500.00Still Can’t Find What You Need?
SBS Genetech EcosystemTM helps you to find life science products from more Chinese suppliers
- ContactFor more information, please fill out the provided form or contact us directly by E-mail
SBS Genetech © Copyright 2000-2024
from China, for the World
for Superior Biology Services since 2000