Description

Plasmids are stable genetic elements outside the chromosome, ranging in size from 1 to 200 kb. They are double-stranded, circular DNA molecules that exist as supercoiled structures in host cells. As the smallest genetic units in lower organisms, plasmids have advantages like low molecular weight and rapid replication, making them indispensable tools in molecular biology experiments.

High-quality plasmids play a crucial role in experimental research, including gene therapy, vaccine preparation, and eukaryotic cell transfection. During the plasmid extraction process, the cell walls of Gram-negative bacteria may release toxic lipopolysaccharides (LPS), also known as endotoxins, due to autolysis or other disruptions. These endotoxins can affect the uptake of plasmid DNA during transfection, reducing transfection efficiency. They can also activate nonspecific immune responses, interfering with the transfection process. Therefore, the purity of plasmids and their endotoxin content largely determine the success of experiments.

Our plasmid DNA preparation services cater not only to the needs of laboratory researchers for small-scale plasmid DNA production but also to bio-industrial users and pharmaceutical companies for large-scale plasmid DNA preparation. Our high-quality plasmids will help customers efficiently complete cell transfection, DNA vaccine development, antibody preparation, and other preclinical studies.

Notes:

• The above prices are for high-copy plasmid extraction. Please inquire for low-copy and large plasmid extraction.
• The above service times are based on normal experimental samples, excluding special or large quantity samples.

Technical Service Advantages

• Comprehensive Plasmid Extraction Levels: We offer research-grade, transfection-grade, and medical-grade plasmid extraction to meet the diverse needs of our clients.
• Strict Quality Control: We provide data including appearance, A260/280 ratio, and supercoiling proportion. We can also offer restriction enzyme analysis, sequencing validation, and endotoxin level testing based on customer requirements.
• Flexible Selection Range: Choose from 100μg to 10mg, with options for high-copy or low-copy plasmids.
• Fast Delivery Time: Medium-scale extraction takes only 3 working days.

Products and Materials Delivered

• Plasmids: Lyophilized plasmid powder or plasmid DNA at specific concentrations to meet the agreed-upon grade and quantity requirements.
• QC Report: Includes data on appearance, A260/280 ratio, supercoiling proportion; restriction enzyme analysis, sequencing validation, and optional endotoxin level testing.

Related: Gene Synthesis Services

SBS Genetech is recognized as one of the global major leading industry players in Gene Synthesis by third-party market researchers. For more details, please visit GENE SYNTHESIS MARKET SIZE & SHARE ANALYSIS - GROWTH TRENDS & FORECASTS (2023 - 2028).