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Human ORF Gene Cloning Service

Human ORF Gene Cloning Service

The SBS Genetech Human Open Reading Frame (ORF) Library comprises approximately 15,000 human ORF clones, including around 12,000 well‑annotated genes. Each ORF represents the protein‑coding region between the start and stop codons, excluding the 5' and 3' untranslated regions (UTRs). All clones are generated by reverse‑transcribing mRNA into cDNA followed by Gateway BP recombination into the pDONR223 entry vector, and are intentionally provided without a stop codon to support flexible downstream tagging and expression. Using Gateway LR reactions or PCR‑based cloning, researchers can readily transfer any ORF into a wide range of expression vectors. Through the Human ORF Gene Cloning Service, SBS Genetech offers rapid amplification and sequence‑verified preparation of any clone from the library within 3–5 business days, with optional services including custom subcloning into user‑specified vectors and large‑scale plasmid preparation.
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Description

The SBS Genetech Human Open Reading Frame (ORF) Library contains approximately 15,000 human gene ORF clones, including around 12,000 well‑annotated genes. An ORF clone refers to the coding sequence between the start and stop codons, excluding the 5' and 3' untranslated regions (UTRs) of mRNA.

Each ORF clone in the library is generated by reverse‑transcribing mRNA into cDNA encoding the target protein, followed by Gateway BP recombination into the pDONR223 entry vector. The recombination site sequence is shown in Figure 1. To support downstream tagging using the Gateway system, all ORF clones are provided without a stop codon. Users can easily transfer the ORF into new expression vectors using Gateway LR reactions or PCR‑based cloning.

SBS Genetech’s Human ORF Gene Cloning Service enables rapid amplification and sequence‑verified preparation of any ORF clone from the library within 3–5 business days. Additional services are available, including subcloning the ORF into user‑specified destination vectors and large‑scale plasmid preparation.

 

Key Advantages

SBS Genetech’s ORF cloning platform offers several key advantages:

  • Extensive ORF Coverage: The SBS Genetech Human ORF Library includes approximately 15,000 human ORF clones, covering around 12,000 well‑annotated genes, ensuring broad accessibility for diverse research needs.
  • Sequence‑Verified Clones: Every ORF clone is delivered with full sequencing validation, guaranteeing accuracy and reliability.
  • Custom Subcloning Options: Clients may specify any destination vector, and SBS Genetech will perform precise subcloning according to project requirements.
  • Flexible Plasmid Preparation: Large‑scale plasmid extraction services ranging from 100 μg to 10 mg are available, supporting both research‑grade and industrial‑scale applications.

 

Delivery Specs

SBS Genetech is committed to delivering high‑quality ORF cloning services and provides the following materials for every project:

  • Plasmid Deliverable: A sequence‑verified plasmid containing the requested ORF clone.
  • Technical Service Report: Complete sequencing validation information documenting the accuracy of the cloned ORF.

 

Related: 

 

SBS Genetech is recognized as one of the global major leading industry players in Gene Synthesis by third-party market researchers. For more details, please visit GENE SYNTHESIS MARKET SIZE & SHARE ANALYSIS - GROWTH TRENDS & FORECASTS (2023 - 2028).

 

 

Only for research and not intended for treatment of humans or animals

 

 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

 

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