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Beijing SBS Genetech Co.,Ltd.
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      • About SBS
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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Product 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Natural Compounds
    • Synthetic Biology
    • Enzymes
  • POCT Solution 
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Freeze-Drying System
    • Lateral Flow System
  • About 
    • About SBS
    • Achievements
    • Ecosystem
    • Legal Statement
  • Contact
  • …  
    • Home
    • Product 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
      • Natural Compounds
      • Synthetic Biology
      • Enzymes
    • POCT Solution 
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
      • Freeze-Drying System
      • Lateral Flow System
    • About 
      • About SBS
      • Achievements
      • Ecosystem
      • Legal Statement
    • Contact
    • Login
Beijing SBS Genetech Co.,Ltd.
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NGS Panel Synthesis

NGS Panel Synthesis

We provide professional synthesis and purification services, with each sequencing primer, adapter, and index undergoing strict quality control to safeguard your experiments. Our services are suitable for NGS (Illumina) platforms, molecular detection, and gene diagnostics. They offer high stability, high purity, precise quantitation, and no cross-contamination, making them an excellent choice for medical testing.
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Description

We provide professional synthesis and purification services, with each sequencing primer, adapter, and index undergoing strict quality control to safeguard your experiments. Our services are suitable for NGS (Illumina) platforms, molecular detection, and gene diagnostics. They offer high stability, high purity, precise quantitation, and no cross-contamination, making them an excellent choice for medical testing.

 

ModificationsDescription
  • Phosphorylation
  • 5' phosphorylation can be used for adapters, cloning, gene construction, and ligase-catalyzed ligation reactions. 3' phosphorylation can be used in experiments to resist 3' exonuclease digestion and to prevent DNA polymerase-catalyzed DNA chain extension reactions.
  • Internal Amino Modification
  • Mainly uses C6-dT aminolinker to add to thymine residues for internal modification. After modification, the amino group is 10 atoms away from the main chain, which can be used for further labeling and enzyme linkage (e.g., alkaline phosphatase). Currently, we provide internal amino modifications mediated dT-Dabcyl, dT-Biotin, and dT-Digoxingenin modifications.
  • 5' Amino Modification
  • Used for the preparation of functionalized oligonucleotides, widely used in DNA microarrays and multiplex diagnostic systems. Currently, we provide two types: 5' C6 amino modification and 5' C12 amino modification. The former can be used to connect compounds that do not affect the function of oligonucleotides even if they are close, while the latter is used for affinity purification group connection and some fluorescent labeling, especially when fluorescence might be quenched due to proximity to the DNA chain.
  • 3' Amino Modification
  • Currently, we provide 3' C6 amino modification. It can be used to design new diagnostic probes and antisense nucleotides, for example, highly sensitive 32P or fluorescein labeling at the 5' end and amino modification at the 3' end for other connections. In addition, 3' modification can inhibit 3' exonuclease digestion, making it useful in antisense experiments.
  • Thiol (5' Thiol)
  • Similar to amino modifications in many ways. Thiol can be used to add various modifications like fluorescent labels and biotin. For example, thiol-linked fluorescent probes can be created in the presence of DYS and maleimide derivatives. 5' thiol modification primarily uses 5'-thiol-modifier C6-CE Phosphoramidite or Thiol-Modifier C6 S-S CE Phosphoramidite. After modifying with 5'-Thiol-Modifier C6-CE, silver nitrate oxidation must remove the protective group (trityl), while Thiol-Modifier C6 S-S CE requires DTT reduction to convert disulfide bonds to thiol.
  • Spacer
  • Spacers provide necessary intervals for oligonucleotide labeling to reduce interaction between the label group and oligonucleotide, mainly used in DNA hairpin structure and double-strand structure research. C3 spacer is mainly used to mimic the three-carbon interval between ribose 3' and 5' hydroxyl groups or to "replace" an unknown base in a sequence. 3'-Spacer C3 is used to introduce a 3' spacer to prevent 3' exonuclease and 3' polymerase action. Spacer 18 is often used to introduce a strong hydrophilic group.
  • Phosphorothioate
  • Phosphorothioate-modified oligonucleotides are mainly used in antisense experiments to prevent degradation by nucleases. You can choose full phosphorothioate, but the Tm value of oligonucleotides decreases as the number of phosphorothioate bases increases. To reduce this effect, 2-5 bases at both ends of the primer can be phosphorothioate-modified, typically choosing 3 bases each at the 5' and 3' ends.
  • Locked Nucleic Acid (LNA)
  • A special bicyclic nucleotide derivative containing one or more 2'-O, 4'-C-methylene-β-D-ribofuranose monomers. The ribose 2'-O position and 4'-C position form a ring through various bridging actions (e.g., methylene, sulfur methylene, or amine methylene bridges). This ring locks the C3'-endo conformation of the furanose, reducing the flexibility of the ribose structure and increasing the local stability of the phosphate backbone. Since LNA shares the same phosphate backbone structure as DNA/RNA, it has excellent recognition capability and strong affinity for DNA and RNA. Compared to other oligonucleotide analogs, LNA offers many advantages: a) Strong thermal stability in complementary double-strands with DNA/RNA (ΔTm=3-8°C); b) Stability against 3' exonuclease degradation; c) Ability to activate RNase H when hybridized with DNA; d) Good water solubility, freely entering cell membranes and easily absorbed by the body; e) No toxicity in vivo; f) Efficient automatic oligo formation, with a relatively simple synthesis method. Partially or fully modified LNA oligo chains can be synthesized on a DNA synthesizer using phosphoramidite chemistry.

 

Service Commitment

  • Provide Thermo LTQ XL high-precision mass spectrometry reports and Waters high-performance liquid chromatography purity detection reports.

  • High-precision mass spectrometry, single main peak, theoretical molecular weight consistent with detected molecular weight; purity greater than 90%.

 

Service Features

  • Ultra-short chain DNA/RNA synthesis, providing synthesis services for DNA/RNA as short as 2 nucleotides.

  • Ultra-long chain DNA/RNA synthesis, providing synthesis services for DNA up to 200 nucleotides and RNA up to 70 nucleotides.

  • Extensive experience with a variety of DNA/RNA modifications.

  • Complete mass spectrometry inspection for modified DNA/RNA synthesis and Waters high-performance liquid chromatography inspection, ensuring reliable use for customers.

  • Flexible synthesis scale, offering synthesis services from microgram to gram-scale.

  • Rapid delivery, with regular modifications shipped within 2-3 working days.

  • Clinical-grade purity greater than 99%, high stability, no cross-contamination.

 

How should oligonucleotides be stored?

  • DNA dry powder is very stable and can be stored at -20℃ for up to 2 years. DNA can be dissolved in sterile water or TE buffer; dissolved DNA should ideally be aliquoted and stored at -20℃ to avoid repeated freeze-thaw cycles. DNA that is repeatedly used within a short term (2-3 weeks) can be stored at 4℃. Avoid storing dissolved DNA at room temperature, and it is not recommended to use DNA that has been at room temperature for over a week.

  • RNA, being much less stable than DNA, should be stored at -80℃, whether in dry powder or solution. If -80℃ storage is not possible, it should be stored at -20℃, and RNA in solution should avoid repeated freeze-thaw cycles. Modified oligonucleotides should be stored at a maximum of -20℃, with fluorescent modifications needing to be stored away from light.

 

 

With over 20 years of leading experience in oligo synthesis, SBS Genetech is recognized as one of the global major leading industry players in Oligonucleotide Synthesis by third-party market researchers. For more details, please visit Oligonucleotide Synthesis Services Market landscape, Top Competitor Analysis, Revenue, Sales With Forecast Data from 2022 to 2028.

 

Selected Published Papers

SBS Genetech supplies high-quality oligonucleotides, empowering researchers to publish over 800 papers in prestigious journals such as Nucleic Acids Research, Biosensors and Bioelectronics, and Blood

Measuring specific interaction of transcription factor ZmDREB1A with its DNA responsive element at the molecular level

Nucleic Acids Research | 01 Jan 2004 | DOI: https://doi.org/10.1093/nar/gnh100

All the DNA sequences were custom synthesized from SBS Genetech Co. Ltd. (Beijing, China). These include the DRE element sequence (ACCGAC), 5′-NH 2 -GATATACT ACCGAC ATGAGTTC-3′, and its complementary ssDNA, 3′-CTATATGATGGCTGTACTCAAG-5′; the DRE element sequence (GCCGAC), 5′-NH 2 -GATATACT GCCGAC ATGAGTTC-3′, and its complementary ssDNA, 3′-CTATATGACGGCTGTACTCAAG-5′; the ERE element (GCCGCC), 5′-NH 2 -CGCAGACATA GCCGCC ATTT-3′, and its complementary ssDNA, 3′-GCGTCTGTATCGGCGGTAAA-5′; the mutant DRE element sequence (ACCGAG), 5′-NH 2 -GATATACT ACCGAG ATGAGTTC-3′, and its complementary ssDNA, 3′-CTATATGATGGCTCTACTCAAG-5′ (the element sequences are underlined).

 

Novel heat shock protein Hsp70L1 activates dendritic cells and acts as a Th1 polarizing adjuvant

Blood | 01 March 2004 | DOI: https://doi.org/10.1182/blood-2003-08-2828

To assess Hsp70L1 expression in DCs, 6-day DCs generated from human peripheral monocytes were stimulated at 5 × 105 cells/mL for 8 hours with 1 μg/mL lipopolysaccharide (LPS) (Sigma), 30 μg/mL CpG oligonucleotides (5′-TCGTCGTTCCCCCCCCCC-CC-3′; SBS Genetech, Beijing, China)

 

A thermodynamic overview of naturally occurring intramolecular DNA quadruplexes

Nucleic Acids Research | 30 Aug 2008 | DOI: https://doi.org/10.1093/nar/gkn543

HPLC purified oligonucleotides were procured from SBS Genetech, China. The concentrations of these oligonucleotides were calculated by extrapolation of tabulated values of the monomer bases and dimers at 25°C using procedures reported earlier

 

Quartz crystal microbalance detection of protein amplified by nicked circling, rolling circle amplification and biocatalytic precipitation

Biosensors and Bioelectronics | 31 Oct 2014 | DOI: https://doi.org/10.1016/j.bios.2014.10.055

Deoxyribonucleoside 5′-triphosphates (dNTPs) mixture and all oligonucleotides as depicted in Table S1 of Supporting information were purchased from SBS Genetech. Co. Ltd. (China).

 

Electrochemical aptasensor based on one-step synthesis of Cu2O@aptamer nanospheres for sensitive thrombin detection

Sensors and Actuators B: Chemical | 10 June 2015 | DOI: https://doi.org/10.1016/j.snb.2015.05.089

Blood sera and aptamer were acquired from SBS Genetech Co. Ltd. (Beijing, China) and has the following sequences: 5′-TCTCTCAGTCCGTGGTAGGGCAGGGTTGGGGTGACT-3′......

 

Carbon-based nanocomposites with aptamer-templated silver nanoclusters for the highly sensitive and selective detection of platelet-derived growth factor

Biosensors and Bioelectronics | 16 Nov 2016 | DOI: https://doi.org/10.1016/j.bios.2016.11.019

Aptamer was obtained from SBS Genetech Co., Ltd. (Beijing, China), and the sequence was: 5′-CAG GCT ACG GCA CGT AGA GCA TCA CCA TGA TCC

 

Fe(III)-based metal–organic framework-derived core–shell nanostructure: Sensitive electrochemical platform for high trace determination of heavy metal ions

Biosensors and Bioelectronics | 17 Mar 2017 | DOI: https://doi.org/10.1016/j.bios.2017.03.014

Aptamer strands were obtained from SBS Genetech Co. Ltd (Beijing, China).

 

A CRISPR/Cas12a-based fluorescence aptasensor for the rapid and sensitive detection of ampicillin

International Journal of Biological Macromolecules | 9 June 2023 | DOI: https://doi.org/10.1016/j.ijbiomac.2023.125211

The oligonucleotides employed in this experiment were procured from Beijing SBS Genetech Co. Ltd. (China). The sequences of the reporter and three aptamers were obtained in accordance with their relevant references [36,37], while crRNA and activator DNA sequences were designed in our laboratory.

 

Smart co-delivery of plasmid DNA and doxorubicin using MCM-chitosan-PEG polymerization functionalized with MUC-1 aptamer against breast cancer

Biomedicine & Pharmacotherapy | 19 March 2024 | DOI: https://doi.org/10.1016/j.biopha.2024.116465

The aptamer used (sequence: 5′-GCCCGCCGTGGCTGGGTCTTCCTTGGTCGGTCTACAAAAAAAAAA-SH-3′) was obtained from SBS Genetech Co. Ltd.

 

Exploring aptamer-aTF sandwich and CRISPR-Cas12a methods for sensitive L-lactate biosensing in human serum and saliva

Sensors and Actuators B: Chemical | 28 November 2024 | DOI: https://doi.org/10.1016/j.snb.2024.137015

The oligonucleotides utilised in this study were obtained from Beijing SBS Genetech Co. Ltd. (Beijing, China). The sequences of the dsDNA, reporter, and aptamers were obtained following their relevant references while gRNA...

 

Nucleic acid-free aptamer-CRISPR/Cas14 biosensor for prosthetic joint infection rapid detection

Sensors and Actuators Reports | 24 March 2025 | DOI: https://doi.org/10.1016/j.snr.2025.100318

Nucleotide sequences were synthesized by Beijing SBS Genetech Co., Ltd.

 

 

Only for research and not intended for treatment of humans or animals
 
 

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

 

 

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

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