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Description

In protein chemical synthesis research, solid-phase synthesis is the most commonly used and convenient method. This technique is also highly effective in synthesizing long-chain peptides. However, as the peptide chain lengthens (e.g., beyond 150 amino acids), the reaction becomes increasingly difficult, leading to the production of target peptides with missing residues. These peptides with missing residues are the primary impurities generated during long peptide synthesis. Leveraging over 20 years of experience in peptide synthesis, SBS Genetech has successfully overcome the challenges of long-chain peptide synthesis, providing high-quality long-chain peptide synthesis services to customers worldwide.

Mechanism

To address the many challenges in long peptide synthesis, SBS Genetech has implemented a series of effective measures and methods:

1. Microwave-assisted synthesis: For amino acids that are difficult to couple during synthesis, we utilize microwave-assisted methods, which significantly enhance efficiency and greatly reduce reaction times, while also minimizing the formation of two major side products.

2. Fragment synthesis: When conventional synthesis methods prove challenging and purification is difficult for certain peptides, we employ fragment synthesis by coupling specific amino acids segments separately and then integrating them into the peptide chain as a whole. This method effectively resolves many synthesis issues.

3. Thioester formation: Thioester-based peptide synthesis involves a chemoselective reaction between a solid-phase synthesized N-terminal Cys peptide and a C-terminal thioester peptide, forming an amide bond to link peptides. This method divides the peptide chain into multiple sequences based on the position of Cys residues, synthesizing them separately and then merging them through liquid-phase coupling reactions. It significantly reduces the time required for long peptide synthesis and notably enhances the purity of the final product, making it particularly suitable for synthesizing long-chain peptides containing Cys residues.

SBS Genetech is committed to meeting customer demands by developing these three advanced methods for long peptide synthesis. These methods effectively address challenges such as coupling difficulties, high impurity levels, and low purity, ensuring robust synthesis even for peptides with challenging sequences and abnormal HPLC profiles. We have mastered mature technologies in long peptide synthesis to deliver superior quality products consistently.

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