Funcbeads™ Anti-FLAG Magnetic Beads are covalently coupled with high-quality Anti-FLAG mouse monoclonal antibody and nano amino magnetic beads. It can specifically bind to FLAG-labeled proteins in the animal, plant, or microbial lysates, serum, ascites, etc., and can be used for immunoprecipitation (IP), Co-IP, or purification of FLAG-labeled fusion proteins or their protein complexes.
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Funcbeads™ Anti-FLAG Magnetic Beads can specifically bind to FLAG(DYKDDDDK)-labeled fusion protein and can be used in immunoprecipitation or purification of FLAG-labeled fusion protein or its protein complex with the help of magnetic separation equipment such as VSep™ Magnetic Separators.
Strong specificity and high binding efficiency: Funcbeads™ Anti-FLAG Magnetic Beads has high antibody binding density and strong specificity for the FLAG-labeled protein. Moreover, the size of Funcbeads™ Anti-FLAG Magnetic Beads is small. So non-specific binding is largely eliminated. The concentration of the magnetic beads is about 10mg/ml, with ≥ 0.6mg/ml FLAG antibody.
Various forms of FLAG-labeled protein available: Funcbeads™ Anti-FLAG Magnetic Beads can specifically bind methionine-modified N-terminal FLAG-labeled protein (Met-FLAG-protein), N-terminal FLAG-labeled protein (FLAG-protein), and C-terminal FLAG-labeled protein (Protein-FLAG).
Rapid binding of the target protein: Funcbeads™ Anti-FLAG Magnetic Beads uses nanomagnetic beads (~ 200nm), with large specific surface area, which is convenient for the rapid and effective binding of antibody and antigen. Generally, the process of antigen-binding can be completed within 10 minutes, and the target protein immunoprecipitation can be completed within 30 minutes.
Multiple elution methods: According to the structure, biological function, and subsequent application of the target protein, the product can be eluted by a variety of elution buffers, including FLAG peptide, acidic, and SDS-PAGE loading buffer. In particular, FLAG peptide after elution does not contain the light chain and heavy chain of antibodies, which can effectively solve the interference problem of the light chain and heavy chain in Western Blot assay after immunoprecipitation.
The pH of Funcbeads™ Anti-FLAG Magnetic Beads should be maintained at 6-8.
Please avoid high-speed centrifugation, drying or freezing.
Do not put the magnetic beads in the magnetic field for a long time, otherwise the magnetic beads may agglomerate.
Funcbeads™ Anti-FLAG Magnetic Beads should be gentlely and fully suspended before use.