5-Bromo-4-chloro-3-indole phosphate p-toluidine salt (BCIP) Powder

BCIP can be used as a substrate for alkaline phosphatase. The reaction product is blue and insoluble in water. For color detection of alkaline phosphatase-labeled molecules, a combination reaction of 5-bromo-4-chloro-3-indolyl phosphate (BCIP) and nitrobluetetrazole (NBT) is commonly used.

NBT Powder

NBT is a chromogenic substrate of alkaline phosphatase (AP) and is converted into indigo/purple products under its action. Alkaline phosphatase hydrolyzes 5-bromo-4-chloro-3-indole phosphate (BCIP) to produce indigo white, which is then reduced by NBT to form insoluble blue BCI.

4-nitrophenylphosphate disodium salt hexahydrate (phosphatase substrate pNPP Powder)

pNPP can be used for colorimetric substrates in alkaline and acidic phosphatase ELISA experiments. The soluble final product produced by the reaction is yellow, and the absorbance at 405nm can be read in an enzyme-linked immunosorbent assay (ELISA) reader.

Only for research and not intended for treatment of humans or animals

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