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from China, for the World
for Superior Biology Services since 2000
from China, for the World
for Superior Biology Services since 2000
Thanks to our molecular enzyme evolution technology platform (in silico Design & in vitro Evolution Workflow) and continuous focus on LAMP isothermal amplification technology, our newly upgraded Bst P DNA/RNA System has pushed LAMP isothermal amplification technology to a new height. At this moment, Bst P DNA/RNA Polymerase is one of the best enzymes known for LAMP isothermal amplification.
The whole Bst P DNA/RNA System contains hot start Aptamer, which ensures that the enzyme activity blocking efficiency is >95% at <30°C, and the enzyme activity is completely released within 1 min at >60°C. This characteristic is conducive to the establishment of a reaction system at room temperature and greatly reduces the non-specific amplification at low temperatures. The heat resistance is further improved to 70°C. Due to the qualitative improvement of amplification performance, the difficulty of LAMP primer screening has been greatly reduced, and it is easier to obtain stable amplification results and reliable terminal products.
The performence of Bst P DNA/RNA System is as follows:
Advanced solution for isothermal amplification
Bst P DNA/RNA Polymerase is an upgraded version of Bst DNA/RNA Polymerase through enzyme electronic re-structure and evolution screening (in silico Design & in vitro Evolution), which is generally used for LAMP or RT-LAMP amplification of DNA or RNA.
Hot Start Proterty
With hot start Aptamer, the enzyme activity blocking efficiency is >95% at <30°C.
Reduced Dimer Effect
With a higher reaction temperature (70°C), primer matching conditions will be more rigorous.
Less Primer Usage
With additional Helicase, the F3/B3 primer in the standard LAMP can be completely removed.
More Visible Result
With Leuco-HNB technology, the color contrast is more obvious after the HNB reaction.
common Bst DNA Polymerase
our Bst P DNA/RNA Polymerase
SBS Genetech's isothermal amplification technology has excellent performance
Compared with PCR, SBS Genetech's Isothermal Amplification Technology has the advantages of Shorter Reaction Time and Easier Program.
While Compared with conventional isothermal amplification, SBS Genetech's Isothermal Amplification Technology has the advantages of Higher Specificity, Better Sensitivity, Lower False Positive Rate, and Clearer Observable Results.
Ready for this new experience?
Look no further than our customized lyophilized microbead services! We offer lyophilized microbeads with customized primers and reaction volumes of 20-100 μl per bead, ensuring that your experiments are optimized to your exact specifications. Don't settle for a one-size-fits-all approach - Contact us today to learn more about how our customized lyophilized bead services can take your research to the next level!
Representative Publications Using SBS Genetech Isothermal Amplification Products
Xue, T. , Ma, Z. , Liu, F. , Du, W. , & An, C. . (2020). Pneumocystis jirovecii colonization and its association with pulmonary diseases: a multicenter study based on a modified loop-mediated isothermal amplification assay. BMC Pulmonary Medicine, 20(1).
Zhao, K. , Hu, R. , Ni, J. , Liang, J. , & Li, C. . (2020). Establishment of a porcine parvovirus (PPV) LAMP visual rapid detection method. Journal of Virological Methods, 284, 113924.
Papadakis, G. , Pantazis, A. K. , Fikas, N. , Chatziioannidou, S. , Tsiakalou, V. , & Michaelidou, K. , et al. (2022). Portable real-time colorimetric LAMP-device for rapid quantitative detection of nucleic acids in crude samples. Scientific Reports, 12(1), 1-15.
Wu, R. , Liu, X. , Guo, B. , Chen, F. , & Wang, X. . (2014). Development of double loop-mediated isothermal amplification to detect listeria monocytogenes in food. Current Microbiology, 69(6), 839-845.
Ji, J. , Du, L. Q. , Xie, Q. M. , Cao, Y. C. , & Bee, Y. Z. . (2009). Rapid diagnosis of duck plagues virus infection by loop-mediated isothermal amplification. Research in Veterinary Science, 87(1), 53-58.
Zhang, S. , Xu, X. , Wu, Q. , & Zhang, J. . (2013). Rapid and sensitive detection of pseudomonas aeruginosain bottled water by loop-mediated isothermal amplification. European Food Research & Technology, 236(1), 209-215.
Xu, X. , Zhang, S. , Wu, Q. , Zhang, J. , Li, F. , & Cheng, J. . (2014). Development and Application of a Loop-Mediated Isothermal Amplification (LAMP) Method for Rapid and Sensitive Detection of Enterococcus faecalis in Drinking Water. Journal of Food Safety, 34(2), 103–110.
Zhang, F. , Shi, Y. , Jiang, K. , Song, W. , Ma, C. , & Xu, Z. , et al. (2014). Rapid detection and quantification of Prorocentrum minimum by loop-mediated isothermal amplification and real-time fluorescence quantitative PCR. Journal of Applied Phycology, 26(3), 1379-1388.
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from China, for the World
for Superior Biology Services since 2000