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    Bst P DNA/RNA System

    At SBS Genetech, we are at the forefront of offering solutions for Loop-mediated Isothermal Amplification (LAMP) based on our world-class platform

  • It's time for Bst P DNA/RNA System

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    Thanks to our molecular enzyme evolution technology platform (in silico Design & in vitro Evolution Workflow) and continuous focus on LAMP isothermal amplification technology, our newly upgraded Bst P DNA/RNA System has pushed LAMP isothermal amplification technology to a new height. At this moment, Bst P DNA/RNA Polymerase is one of the best enzymes known for LAMP isothermal amplification.

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    The whole Bst P DNA/RNA System contains hot start Aptamer, which ensures that the enzyme activity blocking efficiency is >95% at <30°C, and the enzyme activity is completely released within 1 min at >60°C. This characteristic is conducive to the establishment of a reaction system at room temperature and greatly reduces the non-specific amplification at low temperatures. The heat resistance is further improved to 70°C. Due to the qualitative improvement of amplification performance, the difficulty of LAMP primer screening has been greatly reduced, and it is easier to obtain stable amplification results and reliable terminal products.

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    The performence of Bst P DNA/RNA System is as follows:

    • The reaction temperature of the reagent is raised to 70°C, which provides more rigorous primer matching conditions and greatly reduces the primer dimer effect. Under this condition, the difficulty of primer screening is greatly reduced, which is conducive to the primer screening of researchers and developers. In addition, the high-temperature reaction makes the nucleic acid release of the crude sample more sufficient, which is easy to be detected without extraction of nucleic acid, and the inactivation of pathogenic bacteria is more sufficient.

     

    • Helicase (unwinding factor) newly developed by us is added to the reagent so that the formation of the "dumbbell" structure substrate can be completed by Helicase. Therefore, the F3/B3 primer in the standard LAMP can be completely removed. At the same time, Helicase has the function of assisting in strand unwinding, which further reduces the concentration of FIP/BIP primers. This experimental scheme was established by our test and named pLAMP (Premium LAMP).

     

    • HotStart Aptamer with high affinity ensures that the residual enzyme activity is less than 5% below 30°C, and the hot start effect is much higher than that of commercial ones. Aptamer with high affinity makes the reaction system easy to establish and maintain uniform amplification performance.

     

    • The newly developed L-HNB colorimetric technology (Leuco-HNB) solves the problem that the color contrast is not obvious after the HNB reaction. At the same time, the advantages of the tolerance of HNB to buffer and sample are retained. The applicability of this colorimetric method is far better than the visualization method based on pH discoloration.

     

  • Bst P DNA/RNA Polymerase

    Advanced solution for isothermal amplification

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    Bst P DNA/RNA Polymerase is an upgraded version of Bst DNA/RNA Polymerase through enzyme electronic re-structure and evolution screening (in silico Design & in vitro Evolution), which is generally used for LAMP or RT-LAMP amplification of DNA or RNA.

  • Hot Start Proterty

    With hot start Aptamer, the enzyme activity blocking efficiency is >95% at <30°C.

    Reduced Dimer Effect

    With a higher reaction temperature (70°C), primer matching conditions will be more rigorous.

    Less Primer Usage

    With additional Helicase, the F3/B3 primer in the standard LAMP can be completely removed.

    More Visible Result

    With Leuco-HNB technology, the color contrast is more obvious after the HNB reaction.

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    common Bst DNA Polymerase

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    our Bst P DNA/RNA Polymerase

  • Comparison

    SBS Genetech's isothermal amplification technology has excellent performance

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    Compared with PCR, SBS Genetech's Isothermal Amplification Technology has the advantages of Shorter Reaction Time and Easier Program.

     

    While Compared with conventional isothermal amplification, SBS Genetech's Isothermal Amplification Technology has the advantages of Higher Specificity, Better Sensitivity, Lower False Positive Rate, and Clearer Observable Results.

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