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Cat. No.: TENV-200 (for 200U)

Cat. No.: TENV-1k (for 1000U)

The lyophilized Thermostable Endonuclease V is also available by inquiry, which can be transported at room temperature.

Description

Endonuclease V, also known as deoxyinosine 3' endonuclease, is a DNA repair enzyme that recognizes double-stranded DNA or single-stranded DNA containing deoxyinosine (dI) bases, which are the deaminated products of adenine. It cleaves the DNA chain at the first and second phosphodiester bonds downstream of the 3' end of dI, generating a 3' hydroxyl and a 5' phosphate cleavage site. Endonuclease V can also recognize inosine (I) bases in RNA and cleave the RNA chain at the first and second phosphodiester bonds downstream of the I base. It can also recognize DNA structures containing abasic (AP) sites, urea, base mismatches, insertion/deletion mismatches, hairpin structures, unpaired loop structures, folded flaps, and Y-shaped structures, although its recognition ability for the latter is not as strong as the former. Recent reports indicate that Endonuclease V can also recognize inosine (I) bases in RNA and predominantly cleave the RNA chain at the second phosphodiester bond downstream of the I base's 3' end. Our company's Thermostable Endonuclease V is derived from thermophilic microorganisms.

Features

• Thermostable enzyme with activity between 30-85°C.
• Heat inactivation: Addition of 0.2N NaOH to a final concentration, followed by heating at 95°C for 5 minutes. Alternatively, phenol-chloroform extraction can be used.

Applications

• Cleavage of DNA oligonucleotides containing deoxyinosine bases.
• Cleavage of RNA oligonucleotides containing inosine bases.
• Weak cleavage activity on mismatched base pairs (compared to inosine bases), with relatively low binding affinity.

Storage

Store at -20°C.