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tech@sbsbio.com
Beijing SBS Genetech Co.,Ltd.
Beijing SBS Genetech Co.,Ltd.
broken image

from China, for the World

for Superior Biology Services since 2000

  • Home
  • Product 
    • All Products
    • Custom Services
    • Catalog Products
    • Innovative Systems
    • Nucleic Acid Related
    • Natural Compounds
    • Synthetic Biology
    • Enzymes
  • POCT Solution 
    • LAMP
    • RPA
    • CRISPR
    • DNA-Free Enzymes
    • Freeze-Drying System
    • Lateral Flow System
  • About 
    • About SBS
    • Achievements
    • Ecosystem
    • Legal Statement
  • Contact
  • …  
    • Home
    • Product 
      • All Products
      • Custom Services
      • Catalog Products
      • Innovative Systems
      • Nucleic Acid Related
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      • Synthetic Biology
      • Enzymes
    • POCT Solution 
      • LAMP
      • RPA
      • CRISPR
      • DNA-Free Enzymes
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      • Lateral Flow System
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Beijing SBS Genetech Co.,Ltd.
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TEV Protease

TEV Protease

$70.00 - $350.00
TEV protease has (NIa) protease catalytic domain which corresponds to a molecular weight of 27 kDa. It is unique with high specificity and is active at low temperature. The tobacco etch virus (TEV) protease is a useful tool for the removal of fusion tags from recombinant proteins.
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All products have special prices for bulk purchase, please contact for more details if required.

 

Cat. No.: TEV-1k (for 1,000U)

Cat. No.: TEV-5k (for 5,000U)

 

Description

TEV Protease (Tobacco etch virus protease) is a recombinant protease derived from tobacco etch virus (TEV) Nla, which is used to excise affinity tags of purified fusion proteins. TEV Protease has strong site-specificity and can recognize the seven amino acid sequence (Glu-Asn-Leu-Tyr-Phe-Gln-Gly) of EXXYXQ (G/S), most commonly ENLYFQG, whose cleavage site is between glutamine and glycine or serine. The enzyme is active in a wide range of pH 5.5-8.5 and 4-30 °C so that the reaction conditions can be modified according to the target proteins. TEV Protease is expressed and purified from E. coli. TEV Protease has a polyhistidine tag and can be removed by affinity chromatography after the enzyme digestion reaction.

 

Unite definition

In 1×TEV Buffer (50 mM Tris-HCl, pH 8.0, 0.5 mM EDTA, 1 mM DTT), the amount of enzyme required to cleave >85% of the 3 µg control substrate at 30 °C for 1 h is defined as an active unit.

 

Storage

TEV Protease can be stored for 2 years at -80 °C for a long time or for 6 months at -20 °C after sub-packaged to avoid repeated freezing and thawing. 10 x TEV Buffer can be stored at -20 °C.

 

Protocol

If the protein is thermally unstable, incubate at 4 °C for a longer time or increase the amount of enzyme used.

  1. Set up the following reaction system in EP tube:
  2. After mixing the system, incubate at 30 °C. Take 30 μl solution at 1, 2, 4, and 6 h respectively, and place it in a separate EP tube.
  3. Add 20 μl 2 x SDS Loading Buffer to the above EP tube and place it at -20 °C.
  4. Take 30 μl sample for SDA-PAGE analysis.

 

Note

To achieve the best digestion result, please ensure that the recombinant protein is partially or completely purified. 

 

Related: Enhanced TEV Protease

 

 

 

Only for research and not intended for treatment of humans or animals

 

 

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory

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