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Cat. No.: ETEV-1k (for 1,000U)

Cat. No.: ETEV-10k (for 10,000U)

Description

Enhanced TEV Protease is a sequence-purified recombinant TEV protease (Tobacco Etch Virus protease). TEV is a commonly used tool enzyme for cleaving affinity tags from fusion proteins, featuring high site-specificity. It strictly recognizes the seven-amino-acid sequence EXXYXQ↓(G/S), with the cleavage site between glutamine and glycine/serine. The most commonly used amino acid sequence is ENLYFQG, with the most efficient cleavage sequence being ENLYFQS. After cleavage, the TEV protease can be removed using its N-terminal 6xHis tag via Ni-NTA resin, facilitating the purification of the target protein.

Product Information

• Product Name: Enhanced TEV Protease
• Expression System: E. coli
• Nature: Recombinant Protein
• Form: Liquid

Unite definition

The amount of enzyme required to cleave >85% of 3 µg substrate in 1X TEV Buffer (50 mM Tris, 50 mM NaCl, 0.5 mM EDTA, 1 mM DTT, pH 8.0) at 30°C for 1 hour is defined as one activity unit.

Inactivation Conditions

85°C for 5 minutes.

Enzyme Storage Solution

50 mM Tris-HCl, 250 mM NaCl, 1 mM TCEP, 1 mM EDTA, pH 7.5, 50% Glycerol

Storage

-20°C storage. Avoid repeated freeze-thaw cycles. It is recommended to aliquot components upon receipt or first use.

Only for research and not intended for treatment of humans or animals

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