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Cat. No.: T4PDG-1k (for 1KU)

Cat. No.: T4PDG-5k (for 5KU)

Cat. No.: T4PDG-20k (for 20KU)

Description

T4 Endonuclease V (T4 PDG), also known as T4 Endo V or T4 pyrimidine dimer glycosylase (T4 PDG), originates from the T4 bacteriophage. It is a DNA damage repair enzyme with N-glycosylase activity and AP lyase activity. The N-glycosylase activity of T4 Endonuclease V can recognize cis-syn cyclobutane pyrimidine dimers (including T^T, T^C, C^C) caused by ultraviolet radiation, and enzymatically cleave at the 5’-glycosidic bond of the pyrimidine dimer to produce an apyrimidinic (AP) site. The AP lyase activity of T4 Endonuclease V removes the AP site through β-elimination, producing a base gap with a 3’-α,β-unsaturated aldehyde and 5’-phosphate.

Application

Can be used for DNA damage research and single cell gel electrophoresis.

Source

Expressed in and purified from Escherichia coli.

Unit Definition

One unit is defined as the amount of enzyme that catalyzes the conversion of 0.5 µg of UV-irradiated supercoiled pUC19 DNA to > 95% nicked plasmid in a total reaction volume of 20 µl in 30 minutes at 37ºC. Nicking is assessed by agarose gel electrophoresis. The irradiated plasmid contains an average of 3-5 pyrimidine dimers.

Purity

Greater than 95%, with no endonuclease or exonuclease activity other than its own, and no RNase activity.

Inactivation or Inhibition

65ºC for 10 minutes.

Storage

Store at -20ºC, effective for at least two years.

Precautions

• When using this product, it should be kept in an ice box or ice bath. After use, it should be immediately stored at -20ºC.
• This product is for scientific research use only by professionals. It is not intended for clinical diagnosis or treatment, nor for use in food or drugs, and should not be stored in a residential area.
• For your safety and health, please wear a lab coat and disposable gloves while handling.