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Explore Versatile Options with Our Glycerol-Free and Lyophilized Variants
Delight in the flexibility of our product offerings, now available in a glycerol-free version, meticulously crafted to seamlessly integrate with your freeze-drying systems.
Additionally, we present a lyophilized version, a practical solution ensuring stable transportation at room temperature, thereby notably mitigating shipping costs, especially during long-distance transits.
Cat. No.: SPP-200 (for 200U)
Cat. No.: SPP-1K (for 1KU)
Description
Sucrose Phosphorylase belongs to the 13th family of glycoside hydrolases and is also known as glucosylglycerate phosphorylase. It is an enzyme that catalyzes the transfer of a glucose glycosidic bond and can catalyze the synthesis of 1-phosphate-glucose from sucrose and inorganic phosphate. The enzyme primarily uses sucrose and 1-phosphate-glucose as donors and various substances such as polyhydroxyl sugars, sugar alcohols, phenolic hydroxyls, carboxyls, etc., as acceptors, catalyzing the synthesis of various glycosides. Sucrose Phosphorylase catalyzes the phosphorylation reaction of sucrose, producing D-fructose and α-D-glucose-1-phosphate, with the reaction equation: Sucrose + Pi = α-D-glucose-1-phosphate + D-fructose. Our company's Sucrose Phosphorylase has a specific activity of >100 U/mg and has been genetically modified to a certain extent for heat resistance. Our Sucrose Phosphorylase is recombinantly expressed and purified through multiple steps.
Unit Definition
One active unit is defined as the amount of enzyme required to catalyze the production of 1.0 μmole of D-fructose, mediated by sucrose, in 1 minute at 37°C in a 1× Sucrose Phosphorylase reaction buffer system.
Activity assay conditions
1× Sucrose Phosphorylase Buffer: 100mM KH2PO4, 200mM Sucrose, pH 7.6.
Features
Applications
Storage
The minimum shelf life is 2 years at -20°C. Avoid repeated freeze-thaw cycles.
Only for research and not intended for treatment of humans or animals