All products have special prices for bulk purchase, please contact us for more details if required.

Explore Versatile Options with Our Glycerol-Free and Lyophilized Variants

Delight in the flexibility of our product offerings, now available in a glycerol-free version, meticulously crafted to seamlessly integrate with your freeze-drying systems. 

Additionally, we present a lyophilized version, a practical solution ensuring stable transportation at room temperature, thereby notably mitigating shipping costs, especially during long-distance transits.

Cat. No.: SPP-200 (for 200U)

Cat. No.: SPP-1K (for 1KU)

Description

Sucrose Phosphorylase belongs to the 13th family of glycoside hydrolases and is also known as glucosylglycerate phosphorylase. It is an enzyme that catalyzes the transfer of a glucose glycosidic bond and can catalyze the synthesis of 1-phosphate-glucose from sucrose and inorganic phosphate. The enzyme primarily uses sucrose and 1-phosphate-glucose as donors and various substances such as polyhydroxyl sugars, sugar alcohols, phenolic hydroxyls, carboxyls, etc., as acceptors, catalyzing the synthesis of various glycosides. Sucrose Phosphorylase catalyzes the phosphorylation reaction of sucrose, producing D-fructose and α-D-glucose-1-phosphate, with the reaction equation: Sucrose + Pi = α-D-glucose-1-phosphate + D-fructose. Our company's Sucrose Phosphorylase has a specific activity of >100 U/mg and has been genetically modified to a certain extent for heat resistance. Our Sucrose Phosphorylase is recombinantly expressed and purified through multiple steps.

Unit Definition

One active unit is defined as the amount of enzyme required to catalyze the production of 1.0 μmole of D-fructose, mediated by sucrose, in 1 minute at 37°C in a 1× Sucrose Phosphorylase reaction buffer system.

Activity assay conditions

1× Sucrose Phosphorylase Buffer: 100mM KH2PO4, 200mM Sucrose, pH 7.6.

Features

• The reaction temperature can range from 30°C to 65°C, with the optimal reaction temperature being 55°C.
• Exhibits some heat resistance, with less than 30% activity loss after incubation at 60°C for 60 hours.

Applications

• Catalyzing the synthesis of 1-phosphate-glucose from sucrose and inorganic phosphate.
• Using sucrose and 1-phosphate-glucose as donors to catalyze the synthesis of various glycosides from polyhydroxyl sugars, sugar alcohols, phenolic hydroxyls, carboxyls, etc.

Storage

The minimum shelf life is 2 years at -20°C. Avoid repeated freeze-thaw cycles.