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Cat. No.: SAAG-1 (for 1ml)

Cat. No.: SAAG-5 (for 5ml)

Cat. No.: SAAG-20 (for 20ml)

Description

Streptavidin Agarose, also known as Streptavidin Agarose Gel or SA Agarose, is made by covalently coupling high-quality streptavidin (Streptavidin, SA) with highly cross-linked 6% agarose. This product can rapidly, efficiently, sensitively, and specifically bind to biotin-labeled antibodies, nucleic acids, proteins, peptides, lectins, and other molecules. It is primarily used for the isolation and purification of biotin-labeled nucleic acids, antibodies, proteins, or related complexes, and is widely applied in immunoprecipitation (IP), cell sorting, and DNA-protein interaction studies.

Streptavidin has a molecular weight of 55 kDa and can bind to biotin with high specificity. The affinity constant between streptavidin and biotin is Kd = 10^-15 M. Streptavidin is a tetrameric protein that can simultaneously bind four biotin molecules. Purified from Streptomyces avidinii, streptavidin shares a high structural similarity and affinity for biotin with avidin, which is derived from egg white. Unlike avidin, streptavidin is a non-glycosylated protein and is essentially uncharged. Avidin has a pI of approximately 10.5 and is alkaline at neutral pH. Due to its uncharged nature at neutral conditions, streptavidin exhibits significantly lower non-specific binding compared to avidin, resulting in a much lower non-specific background during detection. Consequently, streptavidin is commonly used in biotin detection as a substitute for avidin.

Streptavidin Agarose is widely used in the biomedical field, specifically to bind biotin-labeled antigens or antibodies, serving as a carrier for immunoprecipitation, cell sorting, ELISA, and other assays. It can also bind biotin-labeled DNA or RNA fragments to isolate specific nucleic acid-protein complexes from cell or tissue extracts for studies on protein-nucleic acid interactions. Additionally, it is used with biotin-labeled nucleic acid probes for DNA and RNA hybridization experiments, as well as for the separation and purification of mRNA. Furthermore, it can be utilized to purify single-stranded biotin-labeled DNA oligonucleotides and to isolate biotin-labeled PCR products.

Features

• This product has a high binding capacity. Compared to many similar products, it offers an exceptionally high binding capacity, allowing for the rapid separation and purification of biotin-labeled molecules from complex samples. The Streptavidin Agarose in this product is a 50% gel suspension, with each milliliter containing ≥2 mg of high-quality streptavidin protein, capable of binding approximately 1-3 mg of biotin-labeled BSA, making it highly efficient for experiments such as immunoprecipitation.
• This product has strong specificity. It can specifically bind to biotinylated ligands such as antibodies, nucleic acids, proteins, peptides, and lectins, resulting in high purity of the obtained products. These products can be further used in a range of subsequent analytical tests, including Western blotting, ELISA, Northern blotting, qPCR, and mass spectrometry.

This product is a 50% gel suspension, with a total volume that includes 0.5 ml of gel precipitate per milliliter. If 50 μl of the agarose gel suspension is used for each sample, one milliliter of this product can be used for 20 sample reactions.

Storage

Store at -20ºC for one year of effectiveness. At 4ºC, it remains effective for at least one month.

Precautions

• Before use, this product must be properly and thoroughly resuspended. Gently invert the vial several times to ensure even mixing of the agarose gel; avoid vigorous vortexing or shaking to prevent protein denaturation and gel fragmentation.
• This product contains trace amounts of preservatives. It is recommended to wash the gel three times with an appropriate solution, such as TBS, before use to eliminate potential interference from the preservatives.
• When performing immunoprecipitation or purification, it is advisable to design positive and negative control groups.
• The type, size, and biotinylation method of the target molecules will affect the binding efficiency. It is recommended to determine the appropriate agarose gel amount for each specific application through dilution methods. Consider increasing the agarose gel amount to 2-3 times the molar quantity of the target molecules to ensure sufficient binding.
• Free biotin can reduce the binding capacity of this agarose gel. Therefore, after biotinylating proteins or nucleic acids, it is necessary to remove excess free biotin using methods such as desalting columns.
• Purification of protein samples should be completed as soon as possible after collection, and samples should be kept at 4ºC or in an ice bath to slow down protein degradation or denaturation. To effectively inhibit protein degradation, a suitable mixture of protease inhibitors can be added to the protein samples.
• If centrifugation does not completely remove insoluble materials from the protein samples, the sample solution can be filtered through a 0.45 μm filter membrane. Agarose gel used for elution with acidic solutions or SDS-PAGE cannot be reused. To minimize the loss of streptavidin, regardless of whether the operation is manual or automated, the low pH elution step should not exceed 10 minutes.
• High concentrations of DTT, mercaptoethanol, and guanidine hydrochloride may have some effect on the binding of this product to ligands.
• This product is intended for scientific research use only by qualified personnel and must not be used for clinical diagnosis or treatment, nor for food or pharmaceuticals, and should not be stored in ordinary residential areas.
• For your safety and health, please wear a lab coat and disposable gloves during operation.

Only for research and not intended for treatment of humans or animals

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