Cat. No.: SSDRC-2k (for 2KU)
Cat. No.: SSDRC-20k (for 20KU)
Description
During cyclization, 5' - phosphate group and 3' - OH group are required in the substrate single stranded DNA or RNA. For single stranded substrates larger than 15 nt, ssDNA/RNA CircLigase works efficiently. The enzyme can also catalyze the ligation between DNA or RNA molecules and pre-adenylated DNA junctions. In addition, in the case of excessive ATP, the enzyme can adenosylate DNA/RNA molecules.
Contents
2KU
Unit Definition
One unit is defined as the amount of enzyme required to catalyze 1 pmol of a linear 5′-phosphorylated control oligo (55 mer) into circular ssDNA in 1 hour at 60°C.
Note
Storage
Minimum shelf life is 3 year at -20°C.
Instruction: Protocol
Only for research and not intended for treatment of humans or animals
SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory