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Cat. No.: SCPL-0.2 (for 10mM×0.2ml)

Cat. No.: SCPL-25 (for 25mg)

Cat. No.: SCPL-100 (for 100mg)

Description

Scopoletin functions as an inhibitor of acetylcholinesterase (AChE).

Basic Information

• Aliases: Chysatroic acid; Escoopoletin; Gelseminic acid; Scopoletol; 6-Methoxy-7-hydroxycoumarin
• Source: Erycibe obtusifolia Benth.; Hedyotis diffusa Willd; Scopolia japonica Maxin.
• Compound type: Phenylpropanoids ＞ Coumarins
• Chemical Formula: C₁₀H₈O₄
• Molecular Weight: 192.17
• CAS Number: 92-61-5
• Purity: 98%, HPLC
• Solvent/Solubility: DMSO: ≥ 32 mg/mL (166.52 mM)
• Solution Preparation: 2mg dissolved in 1.04mL DMSO, or 1.92mg dissolved in 1mL DMSO, to prepare a 10mM solution.

Signaling Pathway

In Vitro Study

Scopoletin (SCT) is identified as a potential inhibitor of acetylcholinesterase (AChE). It enhances the K+-stimulated release of ACh from rat frontal cortex synaptosomes, exhibiting a bell-shaped dose-response curve (Emax: 4 μM). Scopoletin inhibits the proliferation of PC3 cells by inducing apoptosis, with IC50 values of (157±25), (154±51), and (294±100) mg/L for PC3, PAA (human lung cancer cell), and HeLa cell proliferation, respectively. It induces a notable time- and concentration-dependent inhibition of PC3 cell proliferation, accompanied by reduced protein content and acid phosphatase activity (ACP) levels in PC3 cells in a concentration-dependent manner. Cells treated with Scopoletin exhibit typical morphological changes of apoptosis under light microscopy, fluorescence microscopy, and transmission electron microscopy. The apoptosis rate increases with Scopoletin concentration, with rates of 0.3%, 2.1%, 9.3%, and 35% for Scopoletin concentrations of 0, 100, 200, and 400 mg/L, respectively, while cells in the G2 phase markedly decrease after treatment with Scopoletin.

In Vivo Study

Scopoletin (2 μg, i.c.v.) enhances T-maze alternation and improves novel object recognition in mice with scopolamine-induced cholinergic deficit. It also alleviates age-related deficits in object memory in 15-18-month-old mice (2 mg/kg, s.c.). Mice administered 2 μg of Scopoletin exhibit an increased alternation rate of 71.3±2.5%.

Clinical Trial

N/A

Storage

Store at -20°C for at least one year. The solid powder is stable at 4°C for at least one month. If dissolved in a non-DMSO solvent, it is recommended to aliquot and store at -80°C for up to six months.

Precautions

• This product may have a certain degree of toxicity to the human body. Please take appropriate precautions to avoid direct contact with the body or inhalation.
• This product is for scientific research use only by professionals and is not intended for clinical diagnosis or treatment, food, or pharmaceutical purposes. It should not be stored in ordinary residential areas.
• For your safety and health, please wear laboratory attire and disposable gloves while handling.

Only for research and not intended for treatment of humans or animals

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SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory