All products have special prices for bulk purchase, please contact for more details if required.

As part of our ongoing product development and performance improvements, our RPA product line has been upgraded to Gen-4. This product has been discontinued as part of this transition, and our latest Gen-4 kits are now available.

Both kits support single-target and multiplex amplification, including RT-RPA for RNA target detection.

• RPA Multiplex Basic Lyophilized Kit (Gen-4) – A versatile amplification reagent system for DNA and RNA targets, suitable for single-target and multiplex assay development with compatible downstream detection methods.

• RPA Multiplex Fluorescent Lyophilized Kit (Gen-4) – Supports DNA and RNA target detection through RPA and RT-RPA, with fluorescence detection and compatibility with lateral flow assays when the appropriate assay configuration is used.

For assistance selecting the right kit for your application, please contact our technical team.

Cat. No.: RIB3-48 (for 48T)

Cat. No.: RIB3-96 (for 96T)

Description

This product is the Gen-3 version of RPA (Recombinase Polymerase Amplification) nucleic acid amplification reagents, featuring the additional introduction of the dUTP-UDG-UGI system to effectively prevent aerosol contamination. Supplied as a ready-to-use lyophilized reagent, it includes all components except primers, probes, Mg²⁺, and the template.

To use, simply dissolve the RPA lyophilized particles in nucleic acid release solution, add the template, primers, and Mg²⁺. The final reaction volume is 25 µL. Under constant temperature conditions of 39 to 42°C, the system specifically recognizes and amplifies nucleic acid fragments of the target sample. Detection and assessment can be performed in conjunction with gel electrophoresis or real-time fluorescence method.

Ideal for rapid DNA amplification and detection.

Composition

Recombinase, Polymerase, ATP, dUTP, dATP, dCTP, dGTP, sucrose-sucrose phosphorylase, UGI, buffer, poly A, MgOAc.

Nucleic acid release solution (Optional): UDG, Tris-OAc, Triton   X-100, EDTA, AFSE-15, TET

Features

• First introduction of the dUTP-UDG contamination prevention system in RPA
• Effectively removes aerosol contamination at 10⁶ copies/mL
• A perfect companion for POCT and CRISPR

Storage

This product is transported and stored at 2~28°C, in a dry, and light-protected environment, with a shelf life of 1 year.

Related:

• Gen-1: RPA Basic Kit (Gen-1), RPA RT Basic Kit (Gen-1), RPA Fluorescent Kit (Gen-1), RPA RT Fluorescent Kit (Gen-1)
• Gen-2: RPA Basic Kit (Gen-2), RPA RT Basic Kit (Gen-2), RPA Fluorescent Kit (Gen-2), RPA RT Fluorescent Kit (Gen-2)
• Gen-3: RPA Basic Kit (Gen-3), RPA RT Basic Kit (Gen-3), RPA Fluorescent Kit (Gen-3), RPA RT Fluorescent Kit (Gen-3)
• Gen-4: RPA Multiplex Fluorescent Lyophilized Kit (Gen-4)

Only for research and not intended for treatment of humans or animals