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Cat. No.: RRL-1 (for 1ml)

Cat. No.: RRL-5 (for 5ml)

Description

Recombinant Renilla Luciferase is a luciferase enzyme derived from Renilla and recombinantly expressed in E. coli. It has a molecular weight of approximately 37 kDa. This enzyme uses coelenterazine as a substrate and, in the presence of molecular oxygen, catalyzes the oxidation of coelenterazine to coelenteramide, emitting bioluminescence with a peak wavelength around 465 nm.

Enzyme Activity Definition

One unit of enzyme activity is defined as the amount of enzyme required to produce one relative light unit (RLU) within 10 seconds at 20-25°C.

Purity and Concentration

Purity ≥95% as determined by SDS-PAGE; concentration 2 mg/mL.

Enzyme Storage Buffer

20 mM Tris-HCl, 1 mM DTT, 50% glycerol, pH 8.0.

Storage

Shipped on wet ice; store at -20°C, with a shelf life of 12 months.