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Cat. No.: RMLIF-10 (for 10μg)

Cat. No.: RMLIF-50 (for 50μg)

Cat. No.: RMLIF-200 (for 200μg)

 

Synonyms    

Prolactin; PRL; Prl


Purity    

>98% by SDS-PAGE and HPLC analyses.


Biological Activity    

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat Nb2-11 cells is less than 1.0ng/ml, corresponding to a specific activity of >1.0×106IU/mg.


Physical Appearance    

Sterile Filtered White lyophilized (freeze-dried) powder.


Formulation    

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.


Endotoxin    

Less than 0.1EU/μg of rRtProlactin as determined by LAL method.


Reconstitution    

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.


Category    

Hormones


Background    

Prolactin, also named PRL and lactotrope is a peptide hormone, encoded by the PRL gene. The structure of prolactin is similar to that of growth hormone and placental lactogen. There are mainly three different forms of prolactin in regard to size: little prolactin, big prolactin and big big prolactin. Little prolactin is the predominant form. Although often associated with human milk production, prolactin plays a wide range of other roles in both humans and other verterbrates. Rat prolactin shares 77%~90%a.a. sequence identity with human and murine prolactin. Recombinant rat prolactin is a 22.6kDa globular protein containing 197 amino acid residues.


Amino Acid Sequence    

LPVCSGGDCQ TPLPELFDRV VMLSHYIHTL YTDMFIEFDK QYVQDREFIA KAINDCPTSS LATPEDKEQA QKVPPEVLLN LILSLVHSWN DPLFQLITGL GGIHEAPDAI ISRAKEIEEQ NKRLLEGIEK IISQAYPEAK GNEIYLVWSQ LPSLQGVDEE SKDLAFYNNI RCLRRDSHKV DNYLKFLRCQ IVHKNNC
 

Only for research and not intended for treatment of humans or animals

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