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Cat. No.: MTS10-10 (for 10μg)

Cat. No.: MTS10-100 (for 100μg)

Cat. No.: MTS10-500 (for 500μg)

Synonyms    

BD14; BD3; DEFB103A; DEFB103B; DEFB3; HBD-3; BD3; BD-3; beta-defensin 103; Beta-defensin 3; DEFB103; DEFB-3; DEFB3DEFB103A; Defensin, beta 103; defensin, beta 103B; Defensin-like protein; hBD-3; HBD3HBP3


Purity    

>95% by SDS-PAGE and HPLC analyses.


Biological Activity    

Fully biologically active when compared to standard. Measured by its antimicrobial activity against E. coli. The ED50 for this effect is typically 4-20μg/ml.


Physical Appearance    

Sterile Filtered White lyophilized (freeze-dried) powder.


Formulation    

Lyophilized from a 0.2μM filtered concentrated solution in PBS, pH7.4.


Endotoxin    

Less than 1EU/μg of rRtBD-3 as determined by LAL method.


Reconstitution    

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.


Category    

Cytokine


Background    

Defensins (alpha and beta) are cationic peptides with antimicrobial activity against Gram-negative and Gram-positive bacteria, fungi, and enveloped viruses. They are 2-6kDa proteins and take important roles in innate immune system. On the basis of their size and pattern of disulfide bonding, mammalian defensins are classified into alpha, beta and theta categories. β-Defensins are expressed on some leukocytes and at epithelial surfaces. They contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Because β-defensins are cationic peptides, they can therefore interact with the membrane of invading microbes, which are negative due to lipopolysaccharides (LPS) and lipoteichoic acid (LTA) found in the cell membrane. Especially, they have higher affinity to the binding site compared to Ca2+ and Mg2+ ions. Furthermore, they can affect the stability of the membrane. Additionally, they are not only have the ability to strengthen the innate immune system but can also enhance the adaptive immune system by chemotaxis of monocytes, T-lymphocytes, dendritic cells and mast cells to the infection site.


Amino Acid Sequence    

KKVYNAVSCM TNGGICWLKC SGTFREIGSC GTRQLKCCKK K


 

Only for research and not intended for treatment of humans or animals

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