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Cat. No.: TNFA-10 (for 10μg)

Cat. No.: TNFA-100 (for 100μg)

Cat. No.: TNFA-1k (for 1mg)

Synonyms    

Vascular Endothelial Growth Factor Isoform 165; Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF

Purity    

>97% by SDS-PAGE and HPLC analyses.

Biological Activity    

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is between 1.0-8.0ng/ml.

Physical Appearance    

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation    

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4, with 0.02% Tween-20.

Endotoxin    

Less than 0.1EU/μg of rHuVEGF165 as determined by LAL method.

Reconstitution    

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

Category    

Cytokine

Background    

Vascular Endothelial Growth Factor is a sub-family of growth factors produced by cells, which stimulates vasculogenesis and angiogenesis. VEGF's normal function is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels. Humans express alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (a.a.) in length. VEGF production can be induced in cells that are not receiving enough oxygen. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. Recombinant human VEGF165 contains 166 amino acids residues and it is a disulfide-linked homodimer. In addition, it shares 88%a.a. with corresponding regions of mouse and rat, 96% with porcine, 95% with canine, and 93% with feline, equine and bovine VEGF, respectively.

Amino Acid Sequence    

MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQENPCGP CSERRKHLFV QDPQTCKCSC KNTDSRCKAR QLELNERTCR CDKPRR

Only for research and not intended for treatment of humans or animals

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