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Cat. No.: TNF-50 (for 50μg)

Cat. No.: TNF-100 (for 100μg)

Cat. No.: TNF-1k (for 1mg)

Description

Tumor necrosis factor alpha (TNF-α), also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and murine TNF-α show approximately 79% homology at the amino acid level and cross-reactivity between the two species. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.

Name    

Recombinant Human TNF-α/TNFSF2, His-tag (Recombinant Human Tumor Necrosis Factor-α/TNFSF2, His-tag; rHuTNF-α/TNFSF2, His-tag)

Synonyms   

Cachetin; DIF; TNF; tnfa; tnf-a; TNFSF1A; TNFSF2; APC1 protein; Cachectin; DIF; TNF, monocyte-derived; TNF-a; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF2TNF superfamily, member 2; tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha

Purity    

>97% by SDS-PAGE and HPLC analyses

Biological Activity    

Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05ng/ml, corresponding to a specific activity of >2.0×107IU/mg in the presence of actinomycin D.

Physical Appearance    

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation    

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.0.

Endotoxin    

Less than 1EU/μg of rHuTNF-α/TNFSF2, His-tag as determined by LAL method.
 

Reconstitution    

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category    

Cytokine

Amino Acid Sequence    

MHHHHHHVRS SSRTPSDKPV AHVVANPQAE GQLQWLNRRA NALLANGVEL RDNQLVVPSE GLYLIYSQVL FKGQGCPSTH VLLTHTISRI AVSYQTKVNL LSAIKSPCQR ETPEGAEAKP WYEPIYLGGV FQLEKGDRLS AEINRPDYLD FAESGQVYFG IIAL