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Cat. No.: IL3-10 (for 10μg)

Cat. No.: IL3-100 (for 100μg)

Cat. No.: IL3-1k (for 1mg)

Synonyms    

c-kit Ligand; KITLG; MGF; SLF; DKFZp686F2250; familial progressive hyperpigmentation 2; FPH2; KIT ligand; Kitl; KL-1; Mast cell growth factor; MGFSHEP7; SCFStem cell factor; SFc-Kit ligand; steel factor

Purity    

>97% by SDS-PAGE and HPLC analyses.

Biological Activity    

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 2ng/ml, corresponding to a specific activity of >5.0×105IU/mg.

Physical Appearance    

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation    

Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.

Endotoxin    

Less than 1EU/μg of rHuSCF as determined by LAL method.

Reconstitution    

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.

Category    

Cytokine

Background    

Stem Cell Factor (SCF) that binds to the c-Kit receptor is produced by fibroblasts and endothelial cells. The soluble and transmembrane forms of the protein are formed by alternative splicing of the same RNA transcript and the presence of both soluble and transmembrane SCF is required for normal hematopoietic function. SCF plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. It also promotes mast cell adhesion, migration, proliferation, and survival. Human SCF shares 79%-87%a.a. sequence identity with canine, feline, mouse, and rat SCF. Furthermore, human SCF is weakly active on mouse cells.

Amino Acid Sequence    

EGICRNRVTN NVKDVTKLVA NLPKDYMITL KYVPGMDVLP SHCWISEMVV QLSDSLTDLL DKFSNISEGL SNYSIIDKLV NIVDDLVECV KENSSKDLKK SFKSPEPRLF TPEEFFRIFN RSIDAFKDFV VASETSDCVV SSTLSPEKDS RVSVTKPFML PPVA

 

Only for research and not intended for treatment of humans or animals

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