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Cat. No.: RMLIF-10 (for 10μg)

Cat. No.: RMLIF-50 (for 50μg)

Cat. No.: RMLIF-200 (for 200μg)

 

别名(Synonyms)    

Recombinant Human N-Ras, 重组人N-Ras蛋白, NRas Proto-Oncogene; Transforming Protein N-Ras; Neuroblastoma Ras Viral (V-Ras) Oncogene Homolog, Neuroblastoma Ras Viral oncogene Homolog; proto-oncogene GTPase; ALPS4; AV095280; N-Ras; HRAS1; NS6


产品简介(Background)    

Beyotime’s recombinant human NRas (rhNRas) was expressed in E.coli and purified, which contain the mature form NRas (2-186aa) with Flag tag (DYKDDDDK) at the N-terminus.
Ras (NRas, HRas and KRas) is the most frequently mutated gene family in cancers，they differ significantly only in the C-terminal 40 amino acids. These Ras genes have GTP/GDP binding and GTPase activity, and their normal function may be as G-like regulatory proteins involved in the normal control of cell growth. Ras directly interacts with and activates several downstream effector pathways including the mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) pathways. Mutations in Ras gene disrupt the guanine exchange cycle, typically by becoming GAP-independent and ‘locking’ Ras in the active, GTP-bound state, thereby activating downstream signaling pathways resulting in tumor cell growth.
NRas oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. Mutations which change amino acid residues 12, 13 or 61 activate the potential of NRas to transform cultured cells and are implicated in a variety of human tumors, such as somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia [1].


产品用途(Applications)    

Recombinant Human NRas is useful in studying NRas interacting proteins, effectors, GAPs (GTPase-activating proteins) and GEFs (Guanine nucleotide-exchange factors). It can also be used as positive control in Western blots.


外观(Physical appearance)    

Liquid


活性(Biological activity)    

The specific activity of NRas was 4.3 nmol/min/mg in GTPase activity assay.


浓度(Concentration)    

1mg/ml


纯度(Purity)    

≥ 95% by SDS-PAGE


储存液(Storage buffer)    

20mM HEPES (pH7.4), 200mM NaCl, 1mM EDTA, 2mM DTT, 5% glycerol

Only for research and not intended for treatment of humans or animals

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