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Cat. No.: RMLIF-10 (for 10μg)

Cat. No.: RMLIF-50 (for 50μg)

Cat. No.: RMLIF-200 (for 200μg)

 

别名(Synonyms)    

KRAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RALD, RASK2, K-ras, KRAS proto-oncogene, GTPase, c-Ki-ras2, OES, c-Ki-ras, K-Ras 2, 'C-K-RAS, K-Ras, Kirsten Rat Sarcoma virus


产品简介
(Background)    

Beyotime’s recombinant human KRas4B (rhKRas4B) was expressed in E.coli and purified, which contain the mature form KRas4B (2-186aa) fusion with 6X His tag (HHHHHH) at the N-terminus.
Ras (KRas, HRas and NRas) is the most frequently mutated gene family in cancers，they differ significantly only in the C-terminal 40 amino acids. These Ras genes have GTP/GDP binding and GTPase activity, and their normal function may be as G-like regulatory proteins involved in the normal control of cell growth. Ras directly interacts with and activates several downstream effector pathways including the mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) pathways. Mutations in Ras gene disrupt the guanine exchange cycle, typically by becoming GAP-independent and ‘locking’ Ras in the active, GTP-bound state, thereby activating downstream signaling pathways resulting in tumor cell growth.
KRas was first identified as a viral oncogene in the Kirsten Rat Sarcoma virus and KRas protein was first found as a p21 GTPase. The protein relays signals from outside the cell to the cell’s nucleus. These signals instruct the cell to grow and divide (proliferate) or to mature and take on specialized functions. The KRas gene encodes two highly related protein isoforms, KRas4B and KRas4A, which consist of 188 and 189 amino acids, respectively, due to different clipping of the fourth exon in mammalian cells. These proteins have different structures in their C-terminal region. KRas4B contains a polybasic stretch of eight lysines and KRas4A presents a palmitoylated cysteine and two polybasic regions. The localization and trafficking of KRas4B relies on the presence of polybasic residues that anchor the protein to the inner leaflet of the plasma membrane, whereas the membrane-targeting signals in KRas4A are two polybasic regions and an additional palmitoyl group, that independently contribute to the plasma membrane localization and signal output. As a result of these differences, the four isoforms show distinct subcellular localization and distribution in membrane microdomains, and generate distinct signaling outputs. Mutations in KRas are associated with a variety of malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal cancer [1].


产品用途
(Applications)    

Recombinant Human KRas4B is useful in studying KRas4B interacting proteins, effectors, GAPs (GTPase-activating proteins) and GEFs (Guanine nucleotide-exchange factors). It can also be used as positive control in Western blots.


外观
(Physical appearance)    

Liquid


活性
(Biological activity)    

The specific activity of KRas4B was 0.93 nmol/min/mg in GTPase activity assay.


浓度
(Concentration)    

1mg/ml


纯度(Purity)    

≥ 95% by SDS-PAGE


储存液
(Storage buffer)    

20mM HEPES (pH7.4), 200mM NaCl, 1mM EDTA, 2mM DTT, 5% glycerol
 

Only for research and not intended for treatment of humans or animals

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