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Cat. No.: RMLIF-10 (for 10μg)

Cat. No.: RMLIF-50 (for 50μg)

Cat. No.: RMLIF-200 (for 200μg)

 

别名(Synonyms)    

KRas, KRAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RALD, RASK2, K-ras, KRAS proto-oncogene, GTPase, c-Ki-ras2, OES, c-Ki-ras, K-Ras 2, 'C-K-RAS, K-Ras, Kirsten Rat Sarcoma virus


产品简介
(Background)    

Beyotime’s recombinant human KRas4B G13D (rhKRas4B G13D) was expressed in E.coli and purified, which mutate the code 12 glycine to cysteine of the mature form KRas4B (2-186aa) fusion with 6X His tag (HHHHHH) at the N-terminus.
KRas gene located on the 12p11.1–12p12.1. The KRas is a member of the small GTPase superfamily, which encodes two highly related protein isoforms, KRas4B and KRas4A. KRas4B and KRas4A consist of 188 and 189 amino acids, respectively, due to different clipping of the fourth exon in mammalian cells. These proteins have different structures in their C-terminal region. KRas4B contains a polybasic stretch of eight lysines and KRas4A presents a palmitoylated cysteine and two polybasic regions. The localization and trafficking of KRas4B relies on the presence of polybasic residues that anchor the protein to the inner leaflet of the plasma membrane, whereas the membrane-targeting signals in KRas4A are two polybasic regions and an additional palmitoyl group, that independently contribute to the plasma membrane localization and signal output.
The term KRas is generally referred to as KRas4B due to the high level of mRNA encoding KRas4B in cells. KRas4B is by far the most studied splice variant and frequently observed across cancer types. A single amino acid substitution is responsible for an activating mutation. 98% of KRas4B mutation are found at codon 12 (G12), codon 13 (G13) or codon 61 (Q61). Alterations in G12, G13, and Q61 usually lead to impaired intrinsic GTPase and change the affinity of effectors and metastatic sites. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma [1].


产品用途
(Applications)    

Recombinant Human KRas4B G13D mutant is useful in studying KRas4B interacting proteins, effectors, GAPs (GTPase-activating proteins) and GEFs (Guanine nucleotide-exchange factors). It can also be used as positive control in Western blots.


外观
(Physical appearance)    

Liquid


活性
(Biological activity)    

The specific activity of KRas4B G13D mutant was 0.93nmol/min/mg in GTPase activity assay.


浓度
(Concentration)    

1mg/ml


纯度(Purity)    

≥ 95% by SDS-PAGE


储存液
(Storage buffer)    

20mM HEPES (pH7.4), 200mM NaCl, 1mM EDTA, 2mM DTT, 5% glycerol

Only for research and not intended for treatment of humans or animals

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