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Cat. No.: MTS10-10 (for 10μg)

Cat. No.: MTS10-100 (for 100μg)

Cat. No.: MTS10-500 (for 500μg)

 

Synonyms    

Lymphocyte-activating factor; LAF; Leukocyte Endogenous Mediator; LEM; Endogenous Pyrogen; EP; Mononuclear Cell Factor; MCF; Hematopoietin-1; IL1; IL-1A; IL1F1; IL1-ALPHA


Purity    

>98% by SDS-PAGE and HPLC analyses.


Biological Activity    

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 1.0pg/ml, corresponding to a specific activity of >1.0×10^9IU/mg.


Physical Appearance    

Sterile Filtered White lyophilized (freeze-dried) powder.


Formulation    

Lyophilized from a 0.2µM filtered concentrated solution in 25mM Tris-HCl, pH8.0.


Endotoxin    

Less than 0.01EU/µg of rHuIL-1α GMP as determined by LAL method.


Reconstitution    

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.


Category    

Cytokine


Background    

Interleukin-1 alpha (IL-1α) is a non-secreted proinflammatory cytokine produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. Both IL-1α and IL-1β binds to the same receptor and has similar identical biological properties. Among various species, the amino acid sequence of mature IL-1α is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. IL-1α recently started to find effective application in cosmetic and dermatological formulations, which allow to significantly harmonizing derma architecture.


Amino Acid Sequence    

SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN YFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA

 

Only for research and not intended for treatment of humans or animals

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