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Cat. No.: RMLIF-10 (for 10μg)

Cat. No.: RMLIF-50 (for 50μg)

Cat. No.: RMLIF-200 (for 200μg)

 

别名(Synonyms)    

HRAS, C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, transforming protein p21, p21ras, Harvey rat sarcoma viral oncogene homolog, HRas proto-oncogene, GTPase, Ki-Ras, c-Ki-ras, KRAS2, KRAS, c-K-ras, RASK2


产品简介
(Background)    

Beyotime’s recombinant human HRas (rhHRas) was expressed in E.coli and purified, which contain the mature form HRas (2-186aa) fusion with 6X His tag (HHHHHH) at the N-terminus.
Ras (HRas, NRas and KRas) is the most frequently mutated gene family in cancers，they differ significantly only in the C-terminal 40 amino acids. These Ras genes have GTP/GDP binding and GTPase activity, and their normal function may be as G-like regulatory proteins involved in the normal control of cell growth. Ras directly interacts with and activates several downstream effector pathways including the mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) pathways. Mutations in Ras gene disrupt the guanine exchange cycle, typically by becoming GAP-independent and ‘locking’ Ras in the active, GTP-bound state, thereby activating downstream signaling pathways resulting in tumor cell growth.
HRas, from "Harvey Rat sarcoma virus", is an enzyme that in humans is encoded by the HRas gene. The HRas gene is located on the short (p) arm of chromosome 11 at position 15.5. HRas activate the RAS–RAF–MEK–ERK pathway. Typical hotspots for HRas mutations are found at codon 12, 13 and 61, resulting in G12C/S, G13R/V and Q61R/L mutations. HRas is a frequently mutated oncogene especially in head and neck cancer, bladder cancer, vulvar squamous cell carcinoma, cutaneous squamous cell carcinoma and lung cancer [1].


产品用途
(Applications)    

Recombinant Human HRas is useful in studying HRas interacting proteins, effectors, GAPs (GTPase-activating proteins) and GEFs (Guanine nucleotide-exchange factors). It can also be used as positive control in Western blots.


外观
(Physical appearance)    

Liquid


活性
(Biological activity)    

The specific activity of HRas was 4.3 nmol/min/mg in GTPase activity assay.


浓度
(Concentration)    

1mg/ml


纯度(Purity)    

≥ 95% by SDS-PAGE


储存液
(Storage buffer)    

20mM HEPES (pH7.4), 200mM NaCl, 1mM EDTA, 2mM DTT, 5% glycerol

Only for research and not intended for treatment of humans or animals

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