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Cat. No.: RFL-1 (for 1ml)

Cat. No.: RFL-5 (for 5ml)

Description

Recombinant Firefly Luciferase is a luciferase enzyme derived from the North American firefly (Photinus pyralis) and recombinantly expressed in E. coli. It has a molecular weight of approximately 62 kDa. This enzyme requires ATP, molecular oxygen, and the heterocyclic compound luciferin to produce light through a two-step reaction. Luciferase is widely used in molecular and cellular biology, primarily for the quantitative detection of ATP using bioluminescent assays or as a crucial reagent in the production of luciferase-based assays and research kits.

Enzyme Activity Definition

One unit of enzyme activity is defined as the amount of enzyme required to produce one relative light unit (RLU) within 10 seconds at 20-25°C.

Purity and Concentration

Purity ≥95% as determined by SDS-PAGE; concentration 1 mg/mL.

Enzyme Storage Buffer

20 mM Tris-HCl, 1 mM DTT, 50% glycerol, pH 8.0.

Storage

Shipped on wet ice; store at -20°C, with a shelf life of 12 months.