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Cat. No.: RMLIF-10 (for 10μg)

Cat. No.: RMLIF-50 (for 50μg)

Cat. No.: RMLIF-200 (for 200μg)

 

Synonyms    

GUS; EcGUS; UID


Purity    

＞95% by SDS-PAGE; no detectable DNase and RNase
Unit definition    One unit is defined as the amount of enzyme that will liberate 1.0µg of phenolphthalein from phenolphthalein glucuronide per hour at pH6.8 at 37ºC (30 minute assay).


Activity    

＞20U/μg


Physical appearance    

Liquid


Formulation    

1mg/ml in 1X PBS, pH7.4.


Endotoxin    

N.A.


Category    

Metabolic enzyme


Description    

β-glucuronidase (GUS) is one of the most important lysosomal enzymes. It specifically hydrolyzes β-linked D-glucuronides to D-glucuronic acid and aglycones. Deficiency of the enzyme in human results in the clinical disorder mucopolysaccharidosis type VII (Sly syndrome) which is characterized by an accumulation in lysosomes of glycosoaminoglycans containing terminal glucuronic acid residues. The gene for GUS was originally isolated from Escherichia coli and is now commercially available in a variety of configurations that link the GUS gene to the promoter. GUS has become a most important and versatile reporter gene. There are two unique features of the E. coli GUS gene that make it so fascinating as a reporter gene: (i) absence of endogenous GUS activity in higher plants, in many yeast, fungi, insects, and some bacteria of commercial and agricultural importance; and (ii) E. coli GUS has a higher pH optimum compared to other GUS enzymes present in animals [1].

Only for research and not intended for treatment of humans or animals

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