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Cat. No.: RAPRO-1 (for 1mg)

Cat. No.: RAPRO-10 (for 10mg)

Description

Aprotinin is a competitive serine protease inhibitor that can inhibit the activity of trypsin, chymotrypsin, and kallikrein. Aprotinin forms a stable complex with proteases, thereby blocking the active site of the enzyme. This binding is reversible, and most aprotinin-protease complexes can be dissociated under extreme pH conditions (>10 or <3.0). Aprotinin binds to proteases on a molar basis. Structurally, aprotinin is a monomeric globular protein derived from bovine lungs, composed of 58 amino acids arranged in a single peptide chain with three disulfide bonds. Recombinant aprotinin is expressed in Escherichia coli, purified by multiple column chromatography, and has an amino acid sequence identical to aprotinin derived from bovine lungs. Recombinant aprotinin possesses the same enzymatic properties as the animal-derived aprotinin and can be used as a substitute for animal-derived aprotinin in various biotechnological processes.

Applications

Recombinant aprotinin has the same enzymatic properties as animal-derived aprotinin and can be used as a substitute for animal-derived aprotinin in various biotechnological processes, such as inhibiting serine protease activity in recombinant protein production and cell culture.

Protein Information

• Physical appearance: White, off-white, or pale yellow powder
• Biological activity: ≥ 3.0 EPU/mg pro.
• Unit definition: The ability to inhibit the activity of one unit of trypsin is defined as one aprotinin enzyme potency unit (EPU).
• Purity: ≥ 95% by HPLC
• Protein content: ≥ 60%
• Formulation: Lyophilized from additive-free solution.
• Recommended usage: Aprotinin binds to proteases on a molar basis. Recommended binding pH is >6.0, and it does not bind under conditions with pH <3.0. It can be directly dissolved in 0.9% NaCl and stored at -20°C after reconstitution.
• Stability: Recombinant aprotinin stored as lyophilized powder at 2-8°C is stable for 24 months.
• Amino acid sequence: MKMSRLCLSV ALLVLLGTLA ASTPGCDTSN QAKAQRPDFC LEPPYTGPCK ARMIRYFYNA KAGLCQPFVY GGCRAKRNNF KSSEDCMRTC GGAIGPWENL. Note: This amino acid sequence is for reference only, and the actual amino acid length or sequence may vary.

Advantage

• Animal-free: Recombinant production without any viral contamination or use of animal-derived materials in the production process.
• Stable quality: Batch production ensures stable and consistent quality across multiple batches.
• High purity: High specific activity and host protein residuals below the limits required for biologics.
• Lyophilized powder: Easy storage and transportation.

Precautions

• This product is intended for scientific research purposes by professionals only. It is not intended for clinical diagnosis or treatment, and should not be used in food or drugs. It should not be stored in residential dwellings.
• For your safety and health, please wear lab attire and disposable gloves when handling.

Storage

Store at 4°C or below, effective for two years. Due to partial inactivation caused by each freeze-thaw cycle, after the initial preparation of the corresponding concentration, it should be aliquoted and stored at -20°C or below to avoid repeated freeze-thaw cycles.

Only for research and not intended for treatment of humans or animals

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