All products have special prices for bulk purchase, please contact for more details if required.

Cat. No.: RB69-1 (for 1mg)

Cat. No.: RB69-5 (for 5mg)

Description

The Rb69 gene 32 protein is a single-stranded DNA-binding protein encoded by the Rb69 bacteriophage gene 32, with a molecular weight of 37 kDa. It is an essential component for DNA replication and repair in the Rb69 bacteriophage. It cooperatively binds to and stabilizes transiently formed single-stranded DNA regions, playing a crucial structural role during Rb69 bacteriophage DNA replication. This protein is also widely used for stabilizing and labeling single-stranded regions for the observation of DNA structure within cells using electron microscopy. The Rb69 gene 32 protein can enhance the digestion reactions of restriction endonucleases, the efficiency of reverse transcription in RT-PCR, and the activity of DNA polymerases. Additionally, it can be employed in recombinase polymerase amplification (RPA) reactions.

Applications

• Enhancing the yield and extension capability of reverse transcription during RT-PCR.
• Increasing the yield and specificity of target fragments in PCR of soil samples.
• Stabilizing and labeling ssDNA structures.

Quality Control 

• Protein Purity Assessment: SDS-PAGE gel electrophoresis is employed to assess protein purity, with a minimum purity requirement of 95%.
• Nuclease Endonuclease Activity Assay: 10 μg of Rb69 gene 32 protein is incubated with 200 ng of supercoiled plasmid DNA at 37°C for 4 hours. Gel electrophoresis using agarose gel is conducted, and less than 10% of the plasmid DNA should be converted into nicked or linear forms.
• Non-specific Nuclease Activity Assay: 10 μg of Rb69 gene 32 protein is incubated with 15 ng of double-stranded DNA fragments at 37°C for 16 hours. Gel electrophoresis using agarose gel should show no change in the double-stranded DNA substrate.
• RNase Activity Assay: 10 μg of Rb69 gene 32 protein is incubated with 500 ng of total RNA at 37°C for 1 hour. Gel electrophoresis using agarose gel should indicate that over 90% of the RNA remains intact.
• Host DNA Residual Detection: Fluorescent quantitative PCR is performed using Escherichia coli 16S rDNA-specific primer probes to detect residual host DNA in 10 μg of Rb69 g.

Inactivation

Incubation at 65°C for 20 minutes can render the protein inactive.

Storage

Only for research and not intended for treatment of humans or animals

Journals Using SBS Genetech Products                                       Universities Using SBS Genetech Products

SBS Genetech is a long-term sponsor of Cold Spring Harbor Laboratory