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Cat. No.: RPPE-50 (for 50T)

Cat. No.: RPPE-200 (for 200T)

Description

Our company exclusively introduces a rapid RNA extraction technology that does not require the use of phenol or chloroform. On top of that, we have independently developed a genomic DNA clearing column technology that effectively removes residual gDNA. The obtained RNA typically doesn't need DNase digestion and can be directly used for reverse transcription PCR and quantitative fluorescent PCR experiments. Our unique lysis solution rapidly disrupts cells and inactivates cellular RNA enzymes. The plant RNA aid agent, PLANTaid, helps bind to polysaccharides and polyphenols and removes them via centrifugation. Following this, the lysis mixture is adjusted with ethanol for RNA binding, which is then adsorbed onto the genomic DNA clearing column. This column also captures and clears residual DNA, after which the RNA is selectively eluted and filtered. The filtered RNA, once conditioned with ethanol, selectively adheres to the silica matrix membrane inside the centrifugal column under high salt conditions. After a series of rapid wash and centrifugation steps, impurities such as cellular metabolites and proteins are removed by protein-removing liquid and wash solution. Finally, the pure RNA is eluted from the silica matrix membrane using low-salt RNase-free H2O.

Features

• Does not utilize toxic reagents like phenol, chloroform, etc., and eliminates the need for ethanol precipitation steps.
• Efficient and streamlined; a single sample operation can typically be completed in 25 minutes, making it the fastest and simplest kit in the world.
• Our unique plant RNA auxiliary agent effectively binds to polysaccharides and polyphenols, enhancing their removal.
• Our exclusive genomic DNA clearing column technology has been successfully developed to effectively eliminate residual gDNA. The resulting RNA usually doesn't require DNase digestion and is suitable for reverse transcription PCR and quantitative fluorescent PCR experiments.
• Extremely versatile, it can extract from a wide range of samples, including cotton, pine needles, holly leaves, grape leaves, and over 100 other samples that have failed extraction with other domestic and international kits.
• Multiple column washes ensure high purity, with a typical OD260/OD280 ratio ranging from 1.9 to 2.2. Virtually no residual DNA ensures it is suitable for RT-PCR, Northern-blot, and various other experiments.

Storage

This kit can be stored at room temperature for 12 months without affecting its performance.