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Cat. No.: PVDF123-1 (for 1 roll)

Cat. No.: PVDF123-5 (for 5* 1 roll)

Description

PVDF Membrane (Ultralow Background) is pre-cut or roll-type blotting membrane fabricated from polyvinylidene fluoride (PVDF) with ultralow background. It is most widely used for protein transfer from gels in immunoblotting (Western blot). Its other applicable scenarios include adsorption assays, amino acid analysis, N-terminal protein sequencing, dot and slot blot detection, glycoprotein staining and lipopolysaccharide analysis.

This PVDF membrane line efficiently suppresses non-specific binding between primary/secondary antibodies and the membrane matrix. Extremely low background signals are generated during ECL chemiluminescence development, delivering an excellent signal-to-noise ratio.

Two pre-cut specifications and a bulk roll package are available for this PVDF membrane series. The pre-cut sizes are 6.8 × 8.5 cm and 8.5 × 13.7 cm. The 6.8 × 8.5 cm sheet matches full-size standard gels, eliminating manual trimming for simpler workflows. The 8.5 × 13.7 cm sheet can be further trimmed to custom sizes for protein transfer. The bulk roll measures 27.5 cm × 3.75 m and requires self-cutting, enabling flexible customization of membrane dimensions according to experimental demands.

Two pore sizes (0.22 μm and 0.45 μm) are provided to fit most immunoblotting experiments. The 0.22 μm PVDF membrane is optimized for proteins with molecular weight below 20 kDa. It effectively prevents leakage of low-molecular-weight proteins and exhibits higher protein binding capacity and sequencing yields compared with the 0.45 μm variant. The 0.45 μm PVDF membrane is the standard option for routine Western blotting and performs best with proteins larger than 20 kDa.

PVDF membranes have multiple advantages over nitrocellulose (NC) membranes. They feature outstanding mechanical strength to resist tearing and curling, low background, broad solvent compatibility and superior staining performance. In addition, properly treated PVDF membranes support repeated target detection after antibody stripping.

Due to the strong hydrophobicity of this PVDF membrane series, pre-soaking in appropriate solvents such as ethanol is required for activation before use, which facilitates efficient protein binding to the membrane.

This PVDF membrane series is compatible with a wide range of staining reagents, including Ponceau S, Coomassie Brilliant Blue, Sypro Ruby, transillumination staining, Toluidine Blue, CPTS, colloidal gold, India ink and Amido Black. Stains such as Ponceau S and CPTS can be completely washed off after visualization, allowing the membrane to be reused for subsequent downstream analysis. In contrast, Coomassie Brilliant Blue, India ink and Amido Black produce irreversible staining, rendering the membrane unsuitable for further experimentation post-staining.

The membranes are optimized for chemiluminescence detection and also compatible with conventional colorimetric staining and radioisotope-based detection workflows.

Storage Conditions

Store at room temperature with a shelf life of 5 years.

Precautions

• Use flat-tipped forceps to handle PVDF membranes, and only grip the membrane edges gently. Avoid scratching, folding or creasing the membrane surface during all operations.
• Owing to high hydrophobicity, the membrane must be activated by soaking in ethanol, methanol or other suitable solvents prior to use. Beyotime’s non-toxic PVDF Membrane Wetting & Activation Solution is recommended. A translucent appearance indicates full activation; if the membrane remains opaque white, continue soaking until fully wetted.
• Once wetted and activated, the membrane must remain hydrated and can be stored in Western transfer buffer or wash buffer. If the membrane dries out accidentally, repeat the full wetting and activation procedure.
• For optimal performance, slowly lower the membrane along the liquid surface during activation to ensure uniform coverage by the wetting solution without trapped air bubbles.
• For short-term re-probing of membranes after chemiluminescence exposure: rinse the membrane with distilled water first, then strip bound antibodies using a suitable Western blot stripping buffer. If antibody stripping cannot be performed immediately, submerge the membrane in TBSTw inside a sealed sampling bag to prevent desiccation, and store the sealed bag at 4 °C for up to one week.
• For research use only by qualified laboratory personnel. This product is not intended for clinical diagnosis or treatment, food or pharmaceutical production, nor residential storage.
• Wear a lab coat and disposable gloves at all times during handling for personal safety and health protection.

Related:

• PVDF Membrane (6.6×8.5cm, 0.22μm)
• PVDF Membrane (8.5×13.2cm, 0.22μm)
• PVDF Membrane (26.5cm×3.75m/roll, 0.22μm)
• PVDF Membrane (6.6×8.5cm, 0.45μm)
• PVDF Membrane (8.5×13.2cm, 0.45μm)
• PVDF Membrane (26.5cm×3.75m/roll, 0.45μm)
• PVDF Membrane (6.6×8.5cm, 0.45μm, Low Background Fluorescence)
• PVDF Membrane (8.5×13.2cm, 0.45μm, Low Background Fluorescence)
• PVDF Membrane (26.5cm×3.75m/roll, 0.45μm, Low Background Fluorescence)
• PVDF Membrane (6.8×8.5cm, 0.22μm, Ultralow Background)
• PVDF Membrane (8.5×13.7cm, 0.22μm, Ultralow Background)
• PVDF Membrane (27.5cm×3.75m/roll, 0.22μm, Ultralow Background)
• PVDF Membrane (6.8×8.5cm, 0.45μm, Ultralow Background)
• PVDF Membrane (8.5×13.7cm, 0.45μm, Ultralow Background)
• PVDF Membrane (27.5cm×3.75m/roll, 0.45μm, Ultralow Background)